| Abnormal mitosis in hypertetraploid cells causes aberrant nuclear morphology in association with H2O2-induced premature senescence. | |
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MedLine Citation:
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PMID: 18618767 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aberrant nuclear morphology, such as nuclei with irregular shapes or fragmented nuclei, is often observed in senescent cells, but its biological significance is not fully understood. My previous study showed that aberrant nuclear morphology in senescent human fibroblasts is attributable to abnormal mitosis in later passages. In this study, the production of abnormal nuclei in association with premature senescence was investigated. Premature senescence was induced by brief exposure of human fibroblasts to hydrogen peroxide (H(2)O(2)), and mitosis was observed by time-lapse microscopy. In addition, cell cycle and nuclear morphology after exposure to H(2)O(2) were also analyzed using a laser scanning cytometer. Time-lapse analysis revealed that the induction of premature senescence caused abnormal mitoses, such as mitotic slippage or incomplete mitosis, especially in later days after H(2)O(2) exposure and often resulted in abnormal nuclear morphology. Analysis by laser scanning cytometer showed significantly higher frequency of abnormal cells with deformed nuclei and abnormal mitotic cells with misaligned chromosomes in a hypertetraploid subpopulation. These results suggest that unstable hypertetraploid cells, formed in association with H(2)O(2)-induced premature senescence, cause abnormal mitosis that leads to aberrant nuclear morphology. |
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Authors:
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Susumu Ohshima |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytometry. Part A : the journal of the International Society for Analytical Cytology Volume: 73 ISSN: 1552-4930 ISO Abbreviation: Cytometry A Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-03 Completed Date: 2008-11-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101235694 Medline TA: Cytometry A Country: United States |
Other Details:
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Languages: eng Pagination: 808-15 Citation Subset: IM |
Affiliation:
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Division of Morphological Science, Biomedical Research Center, Saitama Medical University, Saitama, Japan. sohshima@saitama-med.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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drug effects*,
genetics Cell Nucleus / ultrastructure* Cells, Cultured Fibroblasts / drug effects, ultrastructure Humans Hydrogen Peroxide / pharmacology* Laser Scanning Cytometry Mitosis / drug effects* Oxidative Stress* / genetics Polyploidy* |
| Chemical | |
Reg. No./Substance:
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7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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