Document Detail

Abnormal mitochondrial function in locomotor and respiratory muscles of COPD patients.
MedLine Citation:
PMID:  19129279     Owner:  NLM     Status:  MEDLINE    
Several cellular and molecular alterations have been described in skeletal and respiratory muscles of patients with chronic obstructive pulmonary disease (COPD), but information on potential abnormalities of mitochondrial function is scarce. The aim of the present study was to investigate mitochondrial function in the vastus lateralis (VL) and external intercostalis (EI) of COPD patients. Biopsies from VL and EI were obtained during surgery for lung cancer in 13 patients with mild to moderate COPD (age 68+/-6 yrs, forced expiratory volume in one second (FEV(1)) 66+/-15% predicted) and 19 control subjects (age 67+/-9 yrs, FEV(1) 95+/-18% pred). State 3 and 4 mitochondrial oxygen consumption (V'(O(2),m)), ATP synthesis, citrate synthase, cytochrome oxidase (COX) and complex I-III activities, as well as reactive oxygen species (ROS) production, were determined. In COPD patients, in both muscles, COX activity (VL: COPD 3.0+/-0.8 versus control 2.0+/-0.8; EI: 3.7+/-1.6 versus 2.4+/-0.9 micromol min(-1) mg(-1)) and ROS production (VL: 1,643+/-290 versus 1,285+/-468; EI: 1,033+/-210 versus 848+/-288 arbitrary units) were increased, whereas state 3 V'(O(2),m) was reduced (VL: 2.9+/-0.3 versus 3.6+/-0.4; EI: 3.6+/-0.3 versus 4.1+/-0.4 mmol min(-1) kg(-1)). Skeletal muscle mitochondria of patients with chronic obstructive pulmonary disease show electron transport chain blockade and excessive production of reactive oxygen species. The concurrent involvement of both vastus lateralis and external intercostalis suggests a systemic (rather than a local) mechanism(s) already occurring in relatively early stages (Global Initiative for Chronic Obstructive Lung Disease stage II) of the disease.
L Puente-Maestu; J Pérez-Parra; R Godoy; N Moreno; A Tejedor; F González-Aragoneses; J-L Bravo; F Villar Alvarez; S Camaño; A Agustí
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-07
Journal Detail:
Title:  The European respiratory journal     Volume:  33     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-01     Completed Date:  2009-08-18     Revised Date:  2013-05-23    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1045-52     Citation Subset:  IM    
Servicio de Neumología, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
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MeSH Terms
Adenosine Triphosphate / metabolism
Citrate (si)-Synthase / metabolism
Electron Transport Complex IV / metabolism
Forced Expiratory Volume / physiology
Mitochondria, Muscle / metabolism*
Muscle, Skeletal / metabolism,  physiopathology*
Oxygen Consumption / physiology
Pulmonary Disease, Chronic Obstructive / metabolism,  physiopathology*
Reactive Oxygen Species / metabolism
Respiratory Muscles / metabolism,  physiopathology*
Succinate Cytochrome c Oxidoreductase / metabolism
Reg. No./Substance:
0/Reactive Oxygen Species; 56-65-5/Adenosine Triphosphate; EC 1.-/Succinate Cytochrome c Oxidoreductase; EC Transport Complex IV; EC (si)-Synthase

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