| Abnormal micronuclear telomeres lead to an unusual cell cycle checkpoint and defects in Tetrahymena oral morphogenesis. | |
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MedLine Citation:
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PMID: 18469136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomere mutants have been well studied with respect to telomerase and the role of telomere binding proteins, but they have not been used to explore how a downstream morphogenic event is related to the mutated telomeric DNA. We report that alterations at the telomeres can have profound consequences on organellar morphogenesis. Specifically, a telomerase RNA mutation termed ter1-43AA results in the loss of germ line micronuclear telomeres in the binucleate protozoan Tetrahymena thermophila. These cells also display a micronuclear mitotic arrest, characterized by an extreme delay in anaphase with an elongated, condensed chromatin and a mitotic spindle apparatus. This anaphase defect suggests telomere fusions and consequently a spindle rather than a DNA damage checkpoint. Most surprisingly, these mutants exhibit unique, dramatic defects in the formation of the cell's oral apparatus. We suggest that micronuclear telomere loss leads to a "dynamic pause" in the program of cortical development, which may reveal an unusual cell cycle checkpoint. |
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Authors:
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Karen E Kirk; Christina Christ; Jennifer M McGuire; Arun G Paul; Mithaq Vahedi; Kathleen R Stuart; Eric S Cole |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-05-09 |
Journal Detail:
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Title: Eukaryotic cell Volume: 7 ISSN: 1535-9786 ISO Abbreviation: Eukaryotic Cell Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-10-09 Completed Date: 2008-11-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 101130731 Medline TA: Eukaryot Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1712-23 Citation Subset: IM |
Affiliation:
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Department of Biology, Lake Forest College, Lake Forest, Illinois 60045, USA. kirk@lakeforest.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Micronucleus, Germline / genetics, metabolism* Morphogenesis Mouth / growth & development*, metabolism Telomere / genetics, metabolism* Tetrahymena / cytology, genetics, growth & development*, metabolism |
| Comments/Corrections | |
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