Document Detail


Abnormal hypermethylation and clinicopathological significance of Axin gene in lung cancer.
MedLine Citation:
PMID:  23192643     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Axin is an important negative regulator of Wnt pathway. We have reported that reduced expression of Axin could be detected in lung cancer tissues, but the mechanism is not clear. By analyzing the genomic sequence, we note that Axin gene promoter is rich in CpGs. Little is known about the methylation status of Axin gene in lung cancer. So, nested MSP and RT-PCR were used to study the methylation status and mRNA expression of Axin gene in lung cancer tissues and cell lines. The results showed that hypermethylated Axin gene promoter and reduced mRNA expression level of Axin could be detected in lung cancer tissues but not in their paired autologous normal lung tissues (P < 0.01). The hypermethylated Axin gene promoter significantly correlated with the degree of differentiation (P = 0.03), lymph node metastasis (P = 0.048) and TNM classifications (P = 0.032). Demethylation reagent 5-aza-2-deoxycytidine significantly up-regulate Axin expression in BE1 cells (with hypermethylated Axin gene promoter) but not in H460 cells (with unmethylated Axin gene promoter). MTT (3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and transwell matrigel invasion assay showed that 5-aza-2-deoxycytidine treatment inhibited cell growth and invasion more significantly in BE1 cells than that in H460 cells. Our data indicate that hypermethylated Axin gene significantly correlates with the progression of lung cancer and might serve as a new target of clinical therapy for lung cancer patients in future.
Authors:
Lian-He Yang; Hong-Tao Xu; Qing-Chang Li; Gui-Yang Jiang; Xiu-Peng Zhang; Huan-Yu Zhao; Ke Xu; En-Hua Wang
Related Documents :
11026573 - High level, tissue-specific expression of a modified calcitonin/calcitonin gene-related...
16500513 - A novel gene (cmya3) induced in the heart by angiotensin ii-dependent but not salt-depe...
23303923 - Hearing is normal without connexin30.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-29
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  34     ISSN:  1423-0380     ISO Abbreviation:  Tumour Biol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-05-07     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  749-57     Citation Subset:  IM    
Affiliation:
Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, 110001, Liaoning, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / genetics*,  metabolism,  pathology
Axin Protein / genetics*
Azacitidine / analogs & derivatives,  pharmacology
Base Sequence
Blotting, Western
Carcinoma, Large Cell / genetics*,  metabolism,  pathology
Carcinoma, Squamous Cell / genetics*,  metabolism,  pathology
Cell Adhesion
Cell Movement
Cell Proliferation
DNA Methylation*
Female
Gene Expression Regulation, Neoplastic*
Gene Silencing
Humans
Lung Neoplasms / genetics*,  metabolism,  pathology
Lymphatic Metastasis
Male
Middle Aged
Molecular Sequence Data
Neoplasm Staging
Promoter Regions, Genetic / genetics
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/AXIN1 protein, human; 0/Axin Protein; 0/RNA, Messenger; 320-67-2/Azacitidine; 776B62CQ27/decitabine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Targeted glypican-3 gene transcription inhibited the proliferation of human hepatoma cells by specif...
Next Document:  Network Analysis of Social Changes in a Captive Chimpanzee Community Following the Successful Integr...