Document Detail


Abnormal estrous cyclicity after disruption of endothelial and inducible nitric oxide synthase in mice.
MedLine Citation:
PMID:  10377046     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The roles of nitric oxide (NO) and nitric oxide synthase (NOS) in reproduction were studied by examining the estrous cycle of wild-type (WT) mice, inducible NOS (iNOS)-, and endothelial NOS (eNOS)-knockout mice. We observed an average estrous cycle of 4.8 +/- 0.2 days in WT mice. While we observed no significant influence of iNOS deficiency on cycle length, eNOS-knockout females showed a significantly longer estrous cycle (6.6 +/- 0.6 days; p < 0.03) than WT females, due to an extension of diestrus (p < 0.03). There was no influence of iNOS deficiency on ovulation rate compared with that in WT females; however, eNOS-knockout mice showed a significant reduction (p < 0.05) in ovulatory efficiency relative to WT or iNOS-knockout females. In contrast to WT females, in which the highest level of estradiol (E2) was observed at 1500 h of proestrus, iNOS-knockout females reached a peak of E2 at 1830 h of proestrus. In eNOS-knockout females, the peak of E2 occurred at 1830 h, as in iNOS-knockout mice; however, E2 levels were 5-fold and 3-fold higher (p < 0.05) than levels observed in WT and iNOS-knockout females, respectively. There was no effect of genotype on the plasma LH concentrations at proestrus. On the first day of diestrus, eNOS-knockout females showed significantly higher plasma E2 and progesterone levels (p < 0.05) relative to WT and iNOS-knockout females. The dysfunction in cyclicity, ovulation rate, ovarian morphology, and steroidogenesis in eNOS-knockout female mice strongly supports the concept that eNOS/NO plays critical roles in ovulation and follicular development.
Authors:
A Jablonka-Shariff; S Ravi; A N Beltsos; L L Murphy; L M Olson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biology of reproduction     Volume:  61     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-09-03     Completed Date:  1999-09-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  171-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Diestrus
Estradiol / blood
Estrus / physiology*
Female
Mice
Mice, Knockout
Nitric Oxide Synthase / deficiency,  physiology*
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Organ Size
Ovarian Follicle / physiology
Ovary / anatomy & histology
Ovulation / physiology
Proestrus
Chemical
Reg. No./Substance:
50-28-2/Estradiol; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos2 protein, mouse; EC 1.14.13.39/Nos3 protein, mouse

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