Document Detail

Abnormal eicosanoid pattern by blood leukocytes in gastroduodenal ulcer.
MedLine Citation:
PMID:  15448594     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAIDs) are implicated in several diseases showing altered tissue and leukocyte eicosanoid patterns, such as nasal polyposis and asthma. NSAIDs are also associated with gastrointestinal lesions, but it is unknown whether there is an altered eicosanoid pattern. MATERIAL/METHODS: The ex vivo modulated syntheses of prostaglandin E2 (PGE2) and peptido-leukotrienes (pLT) by leukocytes from 41 patients with gastroduodenal ulcer were compared with those of 61 healthy controls. Samples were incubated with diluent, arachidonic acid, or acetylsalicylic acid. The individual syntheses of PGE2 and pLT were quantified using competitive enzyme-immuno-assays followed by calculation of individual eicosanoid patterns. RESULTS: Controls synthesized approximately 4.9-fold whereas patients only approximately 2.9-fold more PGE2 than pLT due to higher basal synthesis of pLT (67 and 125 pg/ml, respectively). The baseline PGE2/pLT ratio was slightly higher in patients (6.1) than in controls (5.7). The arachidonic acid-induced PGE2/pLT ratio in patients (14.2) was significantly higher than in controls (3.3). The acetylsalicylic acid-induced PGE2/pLT ratio in patients (3.5) was significantly lower than in controls (8.3) due to diminished PGE2 and elevated pLT. Integrated individual PGE2 and pLT values revealed a highly significantly altered eicosanoid pattern score in approximately 95% patients and approximately 12% controls. CONCLUSIONS: There is strong evidence of an altered eicosanoid pattern generated by leukocytes of gastroduodenal ulcer patients, which became obvious upon in vitro modulation by arachidonic or acetylsalicylic acid. The phenomenon of an abnormal eicosanoid pattern in gastroduodenal ulcer is yet not fully understood, but may have implications in pathophysiology and diagnostics.
Hanns W Baenkler; Jürgen Zeus; Jürgen Schenk; Dirk Schäfer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-09-23
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  10     ISSN:  1234-1010     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-06     Completed Date:  2005-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  CR557-62     Citation Subset:  IM    
University Hospital, Department of Medicine III, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.
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MeSH Terms
Eicosanoids / metabolism*
Leukocytes / metabolism*
Middle Aged
Peptic Ulcer / metabolism*
Reg. No./Substance:

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