Document Detail

Abnormal desmoglein expression by squamous cell carcinoma cells.
MedLine Citation:
PMID:  8982400     Owner:  NLM     Status:  MEDLINE    
Abnormal expression of cell adhesion molecules and related proteins has been observed in various carcinoma cells. We compared expression patterns of desmosomal cadherins, E-cadherin, and cytoplasmic plaque proteins of four different human squamous cell carcinoma cell lines and in vivo squamous cell carcinoma cells with those of normal human keratinocytes. Unlike normal human keratinocytes, the squamous cell carcinoma cells, both in culture and in vivo, exhibited diminished or unusual expression of desmoglein 3 and desmoglein 1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively. Abnormal expression of E-cadherin and cytoplasmic plaque proteins such as desmoplakin and plakoglobin was also observed. Western blotting study demonstrated that three squamous cell carcinoma cell lines expressed two desmogleins with a predominant 150 kDa molecule, and a minor 130 kDa one. Although these molecular sizes were similar to those of cultured normal human keratinocytes, the 130 kDa desmoglein, which usually carries pemphigus antigenic epitopes, was weakly or negatively reactive with pemphigus vulgaris serum. One squamous cell carcinoma cell line showed a doublet of 140 and 145 kDa bands in addition to the 130 kDa band. All the carcinoma cell lines constantly expressed desmoglein 2 and desmoglein 3 mRNA, whereas cultured normal human keratinocytes always expressed desmoglein 1 and desmoglein 3 mRNA, with or without desmoglein 2 mRNA. These findings indicate that the squamous cell carcinoma cells revealed abnormal expression of desmoglein isoforms, which may be related to tumor cell kinetics such as cell invasion and metastasis.
H Harada; K Iwatsuki; M Ohtsuka; G W Han; F Kaneko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta dermato-venereologica     Volume:  76     ISSN:  0001-5555     ISO Abbreviation:  Acta Derm. Venereol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-04-03     Completed Date:  1997-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370310     Medline TA:  Acta Derm Venereol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  417-20     Citation Subset:  IM    
Department of Dermatology, Fukushima Medical College, Japan.
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MeSH Terms
Antigens / analysis
Cadherins / analysis
Carcinoma, Squamous Cell / chemistry*
Cell Adhesion Molecules / analysis*
Cytoskeletal Proteins / analysis*
Desmoglein 1
Desmoglein 2
Desmoglein 3
Desmosomes / chemistry*
Keratinocytes / chemistry
Pemphigus / immunology
RNA, Messenger / analysis
Skin Neoplasms / chemistry*
gamma Catenin
Reg. No./Substance:
0/Antigens; 0/Cadherins; 0/Cell Adhesion Molecules; 0/Cytoskeletal Proteins; 0/DSG1 protein, human; 0/DSG2 protein, human; 0/DSP protein, human; 0/Desmoglein 1; 0/Desmoglein 2; 0/Desmoglein 3; 0/Desmogleins; 0/Desmoplakins; 0/RNA, Messenger; 0/gamma Catenin

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