Document Detail


Abnormal beta-catenin gene expression with invasiveness of primary hepatocellular carcinoma in China.
MedLine Citation:
PMID:  11819825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the abnormal expression of beta-catenin gene and its relationship ith invasiveness of primary hepatocellular carcinoma among Chinese people. METHODS: Thirty-four hepatocellular carcinoma (HCC) specimens and adjacent para-cancerous tissues, 4 normal liver tissues were immunohistochemically stained to study subcellular distribution of beta-catenin. Semiquantitive analysis of expression of beta-catenin gene exon 3 mRNA was examined by RT-PCR and in situ hybridization. The relationship between expressions of both beta-catenin protein, mRNA and clinicopathological characteristics of HCC was also analyzed. RESULTS: Immuno-histochemistry showed that all normal liver tissues and para-cancerous tissues examined displayed membranous type staining for beta-catenin protein, occasionally with weak expression in the cytoplasm. While 21 cases (61.8%) of HCC examined showed accumulated type in cytoplasms or nuclei. The accumulated type Labling Index (LI) of cancer tissue and para-cancerous tissue was (59.9 +/- 26.3) and (18.3 +/- 9.7) respectively (P<0.01). Higher accumulated type LI was closely related with invasiveness of HCC. Results of RT-PCR showed the beta-catenin gene exon 3 mRNA Expression Index (EI) of 34 HCCs was higher than that of para-cancerous tissue and normal liver tissue. Using in situ hybridization, the signal corresponding to beta-catenin gene exon 3 mRNA was particularly strong in cytoplasm of HCC when compared with those of para-cancerous and normal liver tissues. Over expression of beta-catenin exon 3 was also found to be correlated with high metastatic potential of HCC. CONCLUSION: Abnormal expression of beta-catenin gene may contribute importantly to the invasiveness of HCC among Chinese people.
Authors:
J Cui; X D Zhou; Y K Liu; Z Y Tang; M H Zile
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  7     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2002-04-04     Completed Date:  2002-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  542-6     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA. cuij@msu.edu
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MeSH Terms
Descriptor/Qualifier:
Cadherins / genetics
Carcinoma, Hepatocellular / genetics,  pathology*,  physiopathology*
China
Cytoskeletal Proteins / analysis,  genetics*
Exons
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry
Liver / chemistry,  pathology
Liver Neoplasms / genetics,  pathology*,  physiopathology*
Neoplasm Invasiveness
RNA, Messenger / analysis
Trans-Activators*
beta Catenin
Chemical
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cadherins; 0/Cytoskeletal Proteins; 0/RNA, Messenger; 0/Trans-Activators; 0/beta Catenin

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