Document Detail


Ableson kinases negatively regulate invadopodia function and invasion in head and neck squamous cell carcinoma by inhibiting an HB-EGF autocrine loop.
MedLine Citation:
PMID:  23146907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Head and neck squamous cell carcinoma (HNSCC) has a proclivity for locoregional invasion. HNSCC mediates invasion in part through invadopodia-based proteolysis of the extracellular matrix (ECM). Activation of Src, Erk1/2, Abl and Arg downstream of epidermal growth factor receptor (EGFR) modulates invadopodia activity through phosphorylation of the actin regulatory protein cortactin. In MDA-MB-231 breast cancer cells, Abl and Arg function downstream of Src to phosphorylate cortactin, promoting invadopodia ECM degradation activity and thus assigning a pro-invasive role for Ableson kinases. We report that Abl kinases have an opposite, negative regulatory role in HNSCC where they suppress invadopodia and tumor invasion. Impairment of Abl expression or Abl kinase activity with imatinib mesylate enhanced HNSCC matrix degradation and 3D collagen invasion, functions that were impaired in MDA-MB-231. HNSCC lines with elevated EGFR and Src activation did not contain increased Abl or Arg kinase activity, suggesting that Src could bypass Abl/Arg to phosphorylate cortactin and promote invadopodia ECM degradation. Src-transformed Abl(-/-)/Arg(-/-) fibroblasts produced ECM degrading invadopodia containing pY421 cortactin, indicating that Abl/Arg are dispensable for invadopodia function in this system. Imatinib-treated HNSCC cells had increased EGFR, Erk1/2 and Src activation, enhancing cortactin pY421 and pS405/418 required for invadopodia function. Imatinib stimulated shedding of the EGFR ligand heparin-binding EGF-like growth factor (HB-EGF) from HNSCC cells, where soluble HB-EGF enhanced invadopodia ECM degradation in HNSCC but not in MDA-MB-231. HNSCC cells treated with inhibitors of the EGFR-invadopodia pathway indicated that EGFR and Src are required for invadopodia function. Collectively, our results indicate that Abl kinases negatively regulate HNSCC invasive processes through suppression of an HB-EGF autocrine loop responsible for activating a EGFR-Src-cortactin cascade, in contrast to the invasion promoting functions of Abl kinases in breast and other cancer types. Our results provide mechanistic support for recent failed HNSCC clinical trials utilizing imatinib.
Authors:
K E Hayes; E L Walk; A G Ammer; L C Kelley; K H Martin; S A Weed
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-12
Journal Detail:
Title:  Oncogene     Volume:  32     ISSN:  1476-5594     ISO Abbreviation:  Oncogene     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-04     Completed Date:  2013-11-22     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  4766-77     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology
Benzamides / pharmacology
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Extracellular Matrix / metabolism
Head and Neck Neoplasms / pathology*
Humans
Intercellular Signaling Peptides and Proteins / metabolism*
Neoplasm Invasiveness / prevention & control*
Piperazines / pharmacology
Protein Kinases / metabolism*
Pyrimidines / pharmacology
src-Family Kinases / metabolism
Grant Support
ID/Acronym/Agency:
GM103488/GM/NIGMS NIH HHS; P20 RR016440/RR/NCRR NIH HHS; P20 RR16440/RR/NCRR NIH HHS; P30 GM103488/GM/NIGMS NIH HHS; P30 RR032138/RR/NCRR NIH HHS; P30 RR032138/RR/NCRR NIH HHS; R01 DE014578/DE/NIDCR NIH HHS; R01 DE014578/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Benzamides; 0/Intercellular Signaling Peptides and Proteins; 0/Piperazines; 0/Pyrimidines; 149176-25-0/heparin-binding EGF-like growth factor; BKJ8M8G5HI/imatinib; EC 2.7.-/Protein Kinases; EC 2.7.10.2/src-Family Kinases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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