Document Detail

Ability of in vitro maturing bovine oocytes to transform sperm nuclei to metaphase chromosomes.
MedLine Citation:
PMID:  1339837     Owner:  NLM     Status:  MEDLINE    
Bovine oocytes at the germinal vesicle stage were inseminated in Brackett & Oliphant's medium with bovine serum albumin, caffeine and heparin. Eight hours after insemination, oocytes were transferred into tissue culture medium-199 containing 10% fetal calf serum and cultured for 5-40 h at 39 degrees C in 5% CO2 in air. The proportions of unpenetrated and penetrated oocytes reaching metaphase II increased as the time of examination increased, reaching 70 and 65% 40 h after transfer, respectively. When oocytes were penetrated by more than four spermatozoa, meiotic maturation was greatly retarded. Sperm nuclei were decondensed in most (81%) penetrated oocytes 5 h after transfer. The decondensed sperm nuclei were recondensed and then transformed to metaphase chromosomes which were morphologically compacted at first but became slightly dispersed later. The formation of the metaphase chromosomes was observed in 86% of penetrated oocytes examined 40 h after transfer, and occurred in all metaphase II oocytes at that time. In oocytes penetrated by more than nine spermatozoa, no such transformation of sperm nuclei was observed. Well-developed male and female pro-nuclei were observed in only three (6%) of 51 oocytes penetrated 40 h after transfer.
L R Abeydeera; K Niwa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  96     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-11-02     Completed Date:  1993-11-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  565-72     Citation Subset:  IM    
Division of Animal Science, Faculty of Agriculture, Okayama University, Japan.
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MeSH Terms
Cell Nucleus / ultrastructure*
Culture Techniques
Fertilization in Vitro*
Meiosis / physiology*
Sperm-Ovum Interactions / physiology*
Spermatozoa / cytology*,  ultrastructure
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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