Document Detail


Ability of hematologic and serum biochemical variables to differentiate gram-negative and gram-positive mastitis in dairy cows.
MedLine Citation:
PMID:  11467599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Medical records of 142 dairy cows with clinical mastitis were examined to determine whether hematologic or serum biochemical results could be used to distinguish between mastitis episodes caused by gram-negative bacteria (n = 78) from those caused by gram-positive bacteria (n = 64). Signalment, historic information, hematologic and serum biochemical results, milk culture results, and outcome (discharged from hospital or died) were obtained from the medical records. Cows with gram-negative mastitis had significantly (P < .01) lower blood leukocyte, segmented neutrophil, monocyte, and lymphocyte counts and had higher blood hemoglobin concentrations and hematocrits than did cows with gram-positive mastitis. Serum urea nitrogen was the only serum biochemical result associated with pathogen type, and it was higher in cows with gram-negative mastitis than in those with gram-positive mastitis. Mortality rate (25% overall) did not differ between groups. Logistic regression indicated that routine hematologic analysis (segmented neutrophil count, monocyte count, and hemoglobin concentration) was an accurate predictor of gram-negative mastitis, with a sensitivity of .93, a specificity of .89, and an overall accuracy of 91%. The values for sensitivity and specificity were higher than those previously reported for clinical tests differentiating mastitis episodes caused by gram-negative bacteria from those caused by gram-positive bacteria. Our results indicate that routine hematologic analysis is useful for predicting pathogen type in dairy cows with clinical mastitis, thereby facilitating treatment decisions.
Authors:
G W Smith; P D Constable; D E Morin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary internal medicine / American College of Veterinary Internal Medicine     Volume:  15     ISSN:  0891-6640     ISO Abbreviation:  J. Vet. Intern. Med.     Publication Date:    2001 Jul-Aug
Date Detail:
Created Date:  2001-07-24     Completed Date:  2001-12-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8708660     Medline TA:  J Vet Intern Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  394-400     Citation Subset:  IM    
Affiliation:
Department of Veterinary Clinical Medicine, University of Illinois, Urbana 61802, USA. gw-smith@uiuc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Chemical Analysis / standards,  veterinary
Blood Urea Nitrogen
Cattle
Dairying
Female
Gram-Negative Bacteria / isolation & purification*
Gram-Positive Bacteria / isolation & purification*
Hematologic Tests / standards,  veterinary
Logistic Models
Mastitis, Bovine / blood,  diagnosis*,  microbiology
Milk / microbiology
Predictive Value of Tests
Records as Topic / veterinary
Retrospective Studies
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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