Document Detail


Aberration of morphogenesis of siliceous frustule elements of the diatom Synedra acus in the presence of germanic acid.
MedLine Citation:
PMID:  18205610     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Addition of germanic acid into the culture medium of the diatom Synedra acus subsp. radians (Kutz.) Skabitsch. had nearly no influence on the culture growth at the Ge/Si molar ratio 0.01, but stopped it at ratios 0.05 and higher. It was shown by mass-spectrometry that at the Ge/Si ratio 0.01 germanium was incorporated in both the cytoplasm and siliceous valves, whereas at Ge/Si 0.05 it was incorporated into the cytoplasm but almost failed to accumulate in the valves. At Ge/Si 0.1 germanium was accumulated in the cytoplasm, but its incorporation into the valves terminated. Studies on the cell morphology by light, epifluorescence, and transmission electron microscopy showed that high concentrations of germanic acid induced disorders in morphogenesis of the siliceous frustule and accumulation of large rhodamine-stainable electron-dense inclusions. Model chemical experiments with over-saturated solutions of silicic acid in the presence of polyallylamine revealed that addition of 5% germanic acid considerably accelerated coagulation of silica. Hence, the toxic effect of germanic acid on diatoms could be caused by changes in coagulation of silica.
Authors:
T A Safonova; V V Annenkov; E P Chebykin; E N Danilovtseva; Ye V Likhoshway; M A Grachev
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  72     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2008-01-21     Completed Date:  2008-02-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1261-9     Citation Subset:  IM    
Affiliation:
Limnological Institute, Siberian Branch of the Russian Academy of Sciences, Irkutsk, 664033, Russia.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport, Active / drug effects
Diatoms / drug effects*,  growth & development,  metabolism*,  ultrastructure
Germanium / pharmacology*
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Silicic Acid / metabolism
Silicon / metabolism
Silicon Dioxide / metabolism*
Chemical
Reg. No./Substance:
1343-98-2/Silicic Acid; 7440-21-3/Silicon; 7440-56-4/Germanium; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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