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Aberrant splicing of Gs alpha transcript in transformed human astroglial and glioblastoma cell lines.
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MedLine Citation:
PMID:  1324476     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alpha subunits of G proteins, which play a vital role in signal transduction, display considerable structural and functional diversity. Point mutations in two forms of alpha subunits, Gs alpha and Gi2 alpha, impairing their GTPase activity, have been detected in endocrine disorders. We report here the presence of truncated Gs alpha transcripts in a human glioblastoma cell line, HS683, and in an SV40-transformed human astroglial cell line, SVG. These transcripts were detected by polymerase chain reaction (PCR) amplification of cDNAs from the cell lines. The truncated Gs alpha transcripts, with deletions in the central region of the molecule, seem to have originated due to aberrant splicing within exonic sequences, which did not conform to the consensus GT/AG splice signals. The presence of a smaller size protein of mol.wt. around 25,000 kd in the SVG and HS683 cell lines, detected by antibodies specific for the C-terminal region of the Gs alpha subunit, seems to be consistent with the presence of truncated Gs alpha transcripts in these cell lines. These aberrantly spliced transcripts, if translated, could synthesize potentially oncogenic Gs alpha subunits deficient in GTPase activity. Whether such molecules, with sometimes relatively large deletions, retain some aspects of their function and are biologically significant remains to be seen.
Authors:
I U Ali; W Reinhold; C Salvador; S Aguanno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  20     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-09-23     Completed Date:  1992-09-23     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4263-7     Citation Subset:  IM    
Affiliation:
Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Astrocytes*
Base Sequence
Blotting, Western
Cell Line, Transformed
GTP-Binding Proteins / genetics*
Glioblastoma / genetics*
Humans
Molecular Sequence Data
Mutation / genetics
Polymerase Chain Reaction
RNA Splicing / genetics*
RNA, Messenger / genetics*,  metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 3.6.1.-/GTP-Binding Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 8 Year: 1992
Volume: 20 Issue: 16
First Page: 4263 Last Page: 4267
ID: 334134
PubMed Id: 1324476

Aberrant splicing of Gs alpha transcript in transformed human astroglial and glioblastoma cell lines.
I U Ali
W Reinhold
C Salvador
S Aguanno
Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.



Article Categories:
  • Research Article


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