| Aberrant response in vitro of hormone-responsive prostate cancer cells to antiandrogens. | |
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MedLine Citation:
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PMID: 2710689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antiandrogens are in use alone and in combination with other agents as hormonal therapy for prostate cancer. We conducted studies on the androgen-responsive human prostate cancer cell line LNCaP to determine the direct effects of three antiandrogens (hydroxyflutamide, RU23908, and cyproterone acetate) on hormone-responsive human prostate cancer cells in culture. Dihydrotestosterone (DHT) stimulated the growth of LNCaP cells in a dose-dependent fashion. These cells contained approximately 31,000 high-affinity (Kd = 9 x 10(-10) M) androgen binding sites per cell. In the absence of any androgenic stimulation, all three antiandrogens tested showed agonistic properties by increasing the cell number and uptake of [3H]-thymidine. Competitive uptake studies using [3H]-R1881, a nonmetabolized androgen, showed that the three antiandrogens inhibited specific R1881 uptake with IC50s of 0.9 x 10(-7) M for hydroxyflutamide, 2 x 10(-7) M for RU23908, and 1 x 10(-7) M for cyproterone acetate. It is not known whether these unexpected agonistic effects are due to an altered receptor, previously unmasked agonistic properties of the antiandrogens, or emergence of a hypersensitive clone of cells. |
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Authors:
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G Wilding; M Chen; E P Gelmann |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Prostate Volume: 14 ISSN: 0270-4137 ISO Abbreviation: Prostate Publication Date: 1989 |
Date Detail:
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Created Date: 1989-06-01 Completed Date: 1989-06-01 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8101368 Medline TA: Prostate Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 103-15 Citation Subset: IM |
Affiliation:
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Breast Cancer Section, National Cancer Institute, Bethesda, Maryland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Androgen Antagonists
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pharmacology* Humans Male Prostatic Neoplasms / metabolism* Tumor Cells, Cultured / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Androgen Antagonists |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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