Document Detail

Aberrant mucin glycoprotein patterns of chronic rhinosinusitis patients with bacterial biofilms.
MedLine Citation:
PMID:  21244730     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Increasingly bacterial biofilms have been implicated in chronic rhinosinusitis (CRS), and conventional treatment methods have failed to completely eradicate biofilms. (1) Terminal sialic acids present on sinus mucosal glycoproteins are targets for bacterial adherence and biofilm formation. (2) A subpopulation of CRS patients is more susceptible to biofilm formation due to aberrant terminal sialic residue distribution patterns of glycoproteins on their mucosa. (3) The higher levels of sialyl transferase (ST)6Gal1 gene expression contribute to the overall aberrant glycosylation patterns on the host mucosa that predispose this patient cohort to developing biofilms. (4) Mucin glycoprotein MUC7 that has known bactericidal activity displays an overall reduced terminal sugar profile in biofilm positive CRS patients.
METHODS: Confocal scanning laser microscopy, glycoarray analysis, real-time polymerase chain reaction of ST6Gal1, neuraminidase assays and multivariate analysis were used to compare production of sialic acid-degrading enzymes in sinus biopsies from biofilm positive and negative CRS patients with mucosa from healthy controls.
RESULTS: Biofilm-positive CRS patients expressed aberrant glycoprotein patterns with terminal sialics of between 70 and 90 kD (stress value = 0.1414). The ST6Gal1 gene was upregulated, and bacteria isolated from these patients exhibit significantly higher neuraminidase activity (p = 0.0343). We detected a significant lack in the overall expression of terminal sugar residues of MUC7 (stress value = 0.088).
CONCLUSIONS: We observed a strong positive correlation between the aberrant terminal sugar patterns in this sub group of CRS patients with biofilms. The innate immunity function of their MUC7 glycoprotein against bacterial invasion may be compromised in CRS patients.
Lorwai Tan; Alkis Psaltis; Leonie M Baker; Mike McGuckin; Karine Rousseau; Peter-John Wormald
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of rhinology & allergy     Volume:  24     ISSN:  1945-8932     ISO Abbreviation:  Am J Rhinol Allergy     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2011-01-19     Completed Date:  2011-04-22     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  101490775     Medline TA:  Am J Rhinol Allergy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  319-24     Citation Subset:  IM    
Department of Surgery–Otorhinolaryngology Head and Neck Surgery, University of Adelaide, The Queen Elizabeth Hospital, Australia.
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MeSH Terms
Bacteria / enzymology,  isolation & purification*
Chronic Disease
Microscopy, Confocal
Mucins / analysis*,  physiology
N-Acetylneuraminic Acid / analysis
Neuraminidase / metabolism
Prospective Studies
Rhinitis / etiology*,  metabolism,  microbiology
Salivary Proteins and Peptides / analysis*,  physiology
Sialyltransferases / genetics
Sinusitis / etiology*,  metabolism,  microbiology
Reg. No./Substance:
0/MUC7 protein, human; 0/Mucins; 0/Salivary Proteins and Peptides; 131-48-6/N-Acetylneuraminic Acid; EC 2.4.99.-/Sialyltransferases; EC alpha 2-6-sialyltransferase; EC

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