Document Detail


Aberrant methylation of heparan sulfate glucosamine 3-O-sulfotransferase 2 genes as a biomarker in colorectal cancer.
MedLine Citation:
PMID:  21187457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The first aim was to determine whether hypermethylation of certain tumor related genes, including 3OST2, CHFR, RUNX3, and p16, could be detected in Japanese colorectal cancer (CRC) patients. The second was to utilize the most frequently hypermethylated genes as biomarkers for early detection of CRC.
MATERIALS AND METHODS: We investigated the aberrant promoter methylation profile of 4 genes in 129 colorectal tumors and corresponding normal mucosa. For the second step, bowel lavage and blood of the main tumor drainage vein from 21 CRC patients were analyzed.
RESULTS: The 3OST2 gene was the most frequently methylated (57%) and was methylated frequently (9/21, 43%) in bowel lavage but rarely (1/21; 4.7%) in the venous drainage. The concordance between 3OST2 methylation in bowel lavage and corresponding tumor samples was 71.4%.
CONCLUSION: These results may show the possibility of early detection for CRC by examining 3OST2 gene methylation status in DNA extracted from stool.
Authors:
Yasuharu Tokuyama; Takao Takahashi; Naoki Okumura; Kenichi Nonaka; Yoshihiro Kawaguchi; Kazuya Yamaguchi; Shinji Osada; Adi Gazdar; Kazuhiro Yoshida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-28     Completed Date:  2011-02-04     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  4811-8     Citation Subset:  IM    
Affiliation:
Department of Surgical Oncology, Gifu University, Graduate School of Medicine, Yanagido 1-1, Gifu 500-8705, Japan.
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MeSH Terms
Descriptor/Qualifier:
Azacitidine / analogs & derivatives,  pharmacology
Cell Cycle Proteins / biosynthesis,  genetics
Cell Line, Tumor
Colorectal Neoplasms / enzymology*,  genetics*,  metabolism
Core Binding Factor Alpha 3 Subunit / biosynthesis,  genetics
DNA Methylation*
Gene Expression Regulation, Neoplastic / drug effects
Genes, p16
HCT116 Cells
Humans
Neoplasm Proteins / biosynthesis,  genetics
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Sulfotransferases / biosynthesis,  genetics*
Tumor Markers, Biological / genetics*
Chemical
Reg. No./Substance:
0/CHFR protein, human; 0/Cell Cycle Proteins; 0/Core Binding Factor Alpha 3 Subunit; 0/Neoplasm Proteins; 0/P16 protein, human; 0/Runx3 protein, human; 0/Tumor Markers, Biological; 320-67-2/Azacitidine; 776B62CQ27/decitabine; EC 2.8.2.-/Sulfotransferases; EC 2.8.2.23/heparin-glucosamine 3-O-sulfotransferase
Comments/Corrections
Retraction In:
Anticancer Res. 2012 Nov;32(11):5138   [PMID:  23155297 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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