Document Detail


Aberrant expression of Notch signaling molecules in patients with immune thrombocytopenic purpura.
MedLine Citation:
PMID:  19603167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the role of Notch signaling pathway in immune thrombocytopenic purpura (ITP), we measured the expression of 11 Notch pathway molecules in ITP patients and evaluated their clinical relevance. Real-time reverse transcriptase polymerase chain reaction results showed there was aberrant expression of some Notch molecules in ITP. Notch1 and Notch3 expression elevated, while Notch2 decreased statistically in ITP patients. As for Notch ligands, only DLL1 was found downregulated in ITP. The expression of Notch target gene, Hes1, was also upregulated. In accordance with the mRNA level, Notch1 and Hes1 protein expression was also found elevated by Western blot. Immunocytochemistry showed that Notch1 expressed highly in the cytomembrane, cytoplasm, and part of cellular nucleus for ITP while weak in cytomembrane for controls, and Hes1 of ITP was found expressed higher in cellular nucleus than that of controls. Our findings suggest that the aberrant expression profile of Notch pathway may be involved in ITP, and blockage of Notch1 pathway is likely a promising therapeutic concept.
Authors:
Daoxin Ma; Jianjian Dai; Xiaojuan Zhu; Shuxin Yan; Ping Zhao; Jingru Zhang; Yuanyuan Zhu; Jianzhi Sun; Jun Peng; Chunyan Ji; Ming Hou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-15
Journal Detail:
Title:  Annals of hematology     Volume:  89     ISSN:  1432-0584     ISO Abbreviation:  Ann. Hematol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2009-12-24     Completed Date:  2010-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107334     Medline TA:  Ann Hematol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  155-61     Citation Subset:  IM    
Affiliation:
Department of Hematology, Qilu Hospital, Shandong University, 107 WenhuaXi Road, Jinan, 250012, People's Republic of China. daoxinma@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Basic Helix-Loop-Helix Transcription Factors / genetics,  metabolism
Blotting, Western
Calcium-Binding Proteins / genetics,  metabolism
Homeodomain Proteins / genetics,  metabolism
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins / genetics,  metabolism
Male
Membrane Proteins / genetics,  metabolism
Middle Aged
Purpura, Thrombocytopenic, Idiopathic / genetics,  metabolism*
Receptor, Notch1 / genetics,  metabolism
Receptor, Notch2 / genetics,  metabolism
Receptors, Notch / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction*
Young Adult
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Calcium-Binding Proteins; 0/Homeodomain Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/NOTCH2 protein, human; 0/NOTCH3 protein, human; 0/Receptor, Notch1; 0/Receptor, Notch2; 0/Receptors, Notch; 134324-36-0/Serrate proteins; 149348-15-2/HES1 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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