Document Detail

Aberrant DNA methylation in ulcerative colitis without neoplasia.
MedLine Citation:
PMID:  18507080     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: DNA methylation has been reported to correlate with the development of colitis associated cancer. We detected the promoter methylation of estrogen receptor gene (ER), TP53, p14, p16, p21 and hMLH1 in ulcerative colitis without neoplasia. METHODOLOGY: A total of 49 specimens from 36 patients, including 36 at rectal inflammatory mucosa and 13 at terminal ileum were obtained by colonoscopic biopsies. Methylation specific polymerase chain reaction were performed to detect the methylation in promoters of the above six genes. RESULTS: Methylation rate of ER promoter was significantly higher in the rectal mucosa than that in the ileum (76.3% vs. 46.2%, P=0.044). Moreover, ER methylation in rectal mucosa was significantly higher in relapse-remitting type compared to one attack only type cases (P=0.008), and also increased in cases longer than 7 years (P=0.036). Methylation rates of p14 or p16 were higher in rectal mucosa than those in the ileum, but the differences were not of statistic significance. Meanwhile, methylation in TP53 promoter was found in only one case, while p21 and hMLH1 methylation were negative in all cases. CONCLUSIONS: Methylation in promoters of ER, p14 and p16 occurs in rectal inflammatory mucosa without neoplasia. Examination of ER methylation in rectal mucosa may be useful for predicting cases at high risk of neoplasia.
Fang-Yu Wang; Tomiyasu Arisawa; Tomomitsu Tahara; Kazuya Takahama; Makoto Watanabe; Ichiro Hirata; Hiroshi Nakano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hepato-gastroenterology     Volume:  55     ISSN:  0172-6390     ISO Abbreviation:  Hepatogastroenterology     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-05-29     Completed Date:  2008-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007849     Medline TA:  Hepatogastroenterology     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  62-5     Citation Subset:  IM    
Department of Gastroenterology, Fujita Health University School of Medicine, Toyoake, Japan.
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MeSH Terms
Adaptor Proteins, Signal Transducing / genetics
Colitis, Ulcerative / genetics*
CpG Islands / genetics*
Cyclin-Dependent Kinase Inhibitor p21 / genetics
DNA Methylation*
Genes, p53 / genetics
Intestinal Mucosa / metabolism*
Middle Aged
Neoplasm Proteins / genetics
Nuclear Proteins / genetics
Receptors, Estrogen / genetics
Tumor Suppressor Protein p14ARF / genetics
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/MLH1 protein, human; 0/Neoplasm Proteins; 0/Nuclear Proteins; 0/P16 protein, human; 0/Receptors, Estrogen; 0/Tumor Suppressor Protein p14ARF

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