Document Detail

Aberrant CYP1A1 induction: discrepancy of CYP1A1 mRNA and aryl hydrocarbon hydroxylase activity in mutant cells of mouse hepatoma line, Hepa-1.
MedLine Citation:
PMID:  8071113     Owner:  NLM     Status:  MEDLINE    
We have isolated new benzo[a]pyrene-resistant clones, cl-21 and cl-32, of the mouse hepatoma line, Hepa-1. CYP1A1-dependent aryl hydrocarbon hydroxylase activity is not inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin or 3-methylcholanthrene in these two cell lines. However, mRNA of CYP1A1 is inducible in cl-21 and cl-32 cells, as in the wild-type cells, in spite of an undetectable level of cytosolic Ah receptor. The cl-21 cDNA of Cyp1a-1 was found to have a single mutation leading to an amino acid substitution from Leu (118) to Arg (118). However, the CYP1A1 protein band was not detected on Western immunoblots. The cDNA of cl-32 was found to have a single mutation leading to an amino acid change from Arg (359) to Trp (359). The presence of the mature protein in cl-32 was confirmed by Western blot analysis. Somatic cell hybridization experiments demonstrated that the phenotype of cl-21 and cl-32 is recessive and that these clones belong to the same complementation group. These data suggest that there may be a non-Ah receptor-mediated mechanism of CYP1A1 induction.
H Kikuchi; M Usuda; I Sagami; S Ikawa; M Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  85     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  710-7     Citation Subset:  IM    
Department of Molecular Genetics, Tohoku University, Sendai.
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MeSH Terms
Aryl Hydrocarbon Hydroxylases / metabolism*
Base Sequence
Benzo(a)pyrene / pharmacology
Blotting, Northern
Blotting, Western
Cytochrome P-450 Enzyme System / genetics*
DNA, Complementary / chemistry
Drug Resistance
Hybrid Cells
Liver Neoplasms, Experimental / enzymology*
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger; 50-32-8/Benzo(a)pyrene; 9035-51-2/Cytochrome P-450 Enzyme System; EC Hydrocarbon Hydroxylases

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