| Aberrant CYP1A1 induction: discrepancy of CYP1A1 mRNA and aryl hydrocarbon hydroxylase activity in mutant cells of mouse hepatoma line, Hepa-1. | |
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MedLine Citation:
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PMID: 8071113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have isolated new benzo[a]pyrene-resistant clones, cl-21 and cl-32, of the mouse hepatoma line, Hepa-1. CYP1A1-dependent aryl hydrocarbon hydroxylase activity is not inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin or 3-methylcholanthrene in these two cell lines. However, mRNA of CYP1A1 is inducible in cl-21 and cl-32 cells, as in the wild-type cells, in spite of an undetectable level of cytosolic Ah receptor. The cl-21 cDNA of Cyp1a-1 was found to have a single mutation leading to an amino acid substitution from Leu (118) to Arg (118). However, the CYP1A1 protein band was not detected on Western immunoblots. The cDNA of cl-32 was found to have a single mutation leading to an amino acid change from Arg (359) to Trp (359). The presence of the mature protein in cl-32 was confirmed by Western blot analysis. Somatic cell hybridization experiments demonstrated that the phenotype of cl-21 and cl-32 is recessive and that these clones belong to the same complementation group. These data suggest that there may be a non-Ah receptor-mediated mechanism of CYP1A1 induction. |
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Authors:
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H Kikuchi; M Usuda; I Sagami; S Ikawa; M Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Japanese journal of cancer research : Gann Volume: 85 ISSN: 0910-5050 ISO Abbreviation: Jpn. J. Cancer Res. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-09-29 Completed Date: 1994-09-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8509412 Medline TA: Jpn J Cancer Res Country: JAPAN |
Other Details:
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Languages: eng Pagination: 710-7 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics, Tohoku University, Sendai. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aryl Hydrocarbon Hydroxylases / metabolism* Base Sequence Benzo(a)pyrene / pharmacology Blotting, Northern Blotting, Western Cytochrome P-450 Enzyme System / genetics* DNA, Complementary / chemistry Drug Resistance Hybrid Cells Liver Neoplasms, Experimental / enzymology* Mice Molecular Sequence Data Mutation* Polymerase Chain Reaction RNA, Messenger / metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/RNA, Messenger; 50-32-8/Benzo(a)pyrene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases |
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