Document Detail


Abcb and Abcc transporter homologs are expressed and active in larvae and adults of zebra mussel and induced by chemical stress.
MedLine Citation:
PMID:  22819804     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Multixenobiotic resistance (MXR) of aquatic invertebrates has so far been associated with cellular efflux activity mediated by P-glycoprotein (ABCB1) and MRP (multidrug resistance protein; ABCC) type ABC (ATP binding cassette) transporters. Expression and activity of an abcb1/Abcb1 homolog has been shown in eggs and larvae of the zebra mussel Dreissena polymorpha. Here we report identification of a partial cDNA sequence of an abcc/Abcc homolog from zebra mussel that is transcribed and active as a cellular efflux transporter in embryos and gill tissue of adult mussels. Transcript expression levels were comparatively low in eggs and sharply increased after fertilization, then maintaining high expression levels in 1 and 2 dpf (days post fertilization) larvae. MK571, a known inhibitor of mammalian ABCC transporters, blocks efflux of calcein-am in larvae and gill tissue as indicated by elevated calcein fluorescence; this indicates the presence of active Abcc protein in cells of the larvae and gills. Dacthal and mercury used as chemical stressors both induced expression of abcb1 and abcc mRNAs in larvae; accordingly, assays with calcein-am and ABCB1 inhibitor reversin 205 and ABCC inhibitor MK571 indicated enhanced Abcb1 and Abcc efflux activities. Responses to chemicals were different in gills, where abcb1 transcript abundances were enhanced in dacthal and mercury treatments, whereas abcc mRNA was only increased with mercury. Abcb1 and Abcc activities did not in all cases show increases that were according to respective mRNA levels; thus, Abcc activity was significantly higher with dacthal, whereas Abcb1 activity was unchanged with mercury. Our data indicate that abcb1/Abcb1 and abcc/Abcc transporters are expressed and active in larvae and adult stages of zebra mussel. Expression of both genes is induced as cellular stress response, but regulation appears to differ in larvae and tissue of adult stages.
Authors:
Anna Navarro; Susann Weißbach; Melissa Faria; Carlos Barata; Benjamin Piña; Till Luckenbach
Related Documents :
15189324 - Appetite regulatory neuropeptides are expressed in the sheep hypothalamus before birth.
11517194 - Expression of crhr1 and crhr2 in mouse pituitary and adrenal gland: implications for hp...
15142984 - Promoter analysis of human corticotropin-releasing factor (crf) type 1 receptor and reg...
22916154 - Analysis of mdm2 and mdm4 single nucleotide polymorphisms, mrna splicing and protein ex...
14600494 - P-glycoprotein and caveolin-1alpha in endothelium and astrocytes of primate brain.
24482044 - Microrna‑34a/c function as tumor suppressors in hep‑2 laryngeal carcinoma cells and...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-28
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  122-123C     ISSN:  1879-1514     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  144-152     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differential cognitive effects of energy drink ingredients: Caffeine, taurine, and glucose.
Next Document:  Absorption, distribution, and elimination of graded oral doses of methylmercury in juvenile white st...