Document Detail

Aaptamine, a spongean alkaloid, activates p21 promoter in a p53-independent manner.
MedLine Citation:
PMID:  16480688     Owner:  NLM     Status:  MEDLINE    
Aaptamine, a benzonaphthyridine alkaloid was isolated from a marine sponge on the guidance of a bioassay using the transfected human osteosarcoma MG63 cells (MG63luc(+)). Aaptamine activated p21 promoter stably transfected in MG63 cells dose-dependently at the concentrations of 20-50microM. Expression of p21 and its mRNA in the wild-type MG63 cells also increased by aaptamine-treatment. Furthermore, the cell cycle of MG63 cells was arrested at the G2/M phase within 48h by the aaptamine-treatment. To analyze a responsive element of p21 promoter in the up-regulation of p21 by aaptamine, MG63 cells were transiently transfected with a series of the deleted or mutated promoter segments, and induction of luciferase with aaptamine treatment was examined by using these corresponding transfected cells. The activation of p21 promoter by aaptamine was led through acting Sp1 sites between -82 and -50bp in a p53-independent manner.
Shunji Aoki; Dexin Kong; Hideaki Suna; Yoshihiro Sowa; Toshiyuki Sakai; Andi Setiawan; Motomasa Kobayashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-03
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  342     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-04-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  101-6     Citation Subset:  IM    
Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka 1-6, Suita, Osaka 565-0871, Japan.
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MeSH Terms
Cell Cycle / drug effects
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p21 / genetics*
Gene Deletion
Gene Expression Regulation / drug effects
Genes, Reporter / genetics
Molecular Structure
Mutation / genetics
Naphthyridines / chemistry,  pharmacology*
Porifera / chemistry*,  metabolism
Promoter Regions, Genetic / genetics*
RNA, Messenger / genetics
Tumor Suppressor Protein p53 / metabolism
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor p21; 0/Naphthyridines; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; 85547-22-4/aaptamine

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