Document Detail


AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response.
MedLine Citation:
PMID:  11884684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mutations in the AXR1 gene result in a reduction in auxin response and diverse defects in auxin-regulated growth and development. In a previous study, we showed that AXR1 forms a heterodimer with the ECR1 protein. This enzyme activates the ubiquitin-related protein RUB1 in vitro. Furthermore, we showed that the Skp1-Cul1/Cdc53-F-box (SCF) subunit AtCUL1 is modified by RUB1 in vivo. In this report, we demonstrate that the formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 in vivo. The expression of AXR1 and ECR1 is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s that are required for auxin response.
Authors:
Juan C del Pozo; Sunethra Dharmasiri; Hanjo Hellmann; Loni Walker; William M Gray; Mark Estelle
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Plant cell     Volume:  14     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-03-08     Completed Date:  2002-06-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  421-33     Citation Subset:  IM    
Affiliation:
Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Texas 78712, USA.
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MeSH Terms
Descriptor/Qualifier:
2,4-Dichlorophenoxyacetic Acid / pharmacology
Arabidopsis / drug effects,  genetics*,  growth & development
Arabidopsis Proteins / genetics*,  metabolism
Cell Nucleus / metabolism
Gene Expression Regulation, Plant / drug effects
Growth Substances*
Indoleacetic Acids / pharmacology*
Meristem / genetics,  metabolism
Mutation
Peptide Synthases / genetics
Plant Proteins / genetics*
SKP Cullin F-Box Protein Ligases
Ubiquitins
Grant Support
ID/Acronym/Agency:
R01-GM43644/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/AXR1 protein, Arabidopsis; 0/Arabidopsis Proteins; 0/ECR1 protein, Arabidopsis; 0/Growth Substances; 0/Indoleacetic Acids; 0/Plant Proteins; 0/RUB1 protein, Arabidopsis; 0/Ubiquitins; 94-75-7/2,4-Dichlorophenoxyacetic Acid; EC 6.3.2.-/Peptide Synthases; EC 6.3.2.19/SKP Cullin F-Box Protein Ligases
Comments/Corrections

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