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AW551984: a novel regulator of cardiomyogenesis from pluripotent embryonic cells.
MedLine Citation:
PMID:  21554246     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An understanding of the mechanism that regulates the cardiac differentiation of pluripotent stem cells is necessary for the effective generation and expansion of cardiomyocytes as cell therapy products. Here we have identified genes that modulate the cardiac differentiation of pluripotent embryonic cells. We isolated P19CL6 cell sublines that possess distinct properties in cardiomyogenesis and extracted 24 cardiomyogenesis-related candidate (CMR) genes correlated with cardiomyogenesis using a transcriptome analysis. Knockdown of the CMR genes by RNAi revealed that 18 CMR genes influence spontaneous contraction or transcript levels of cardiac marker genes in embryonal carcinoma (EC) cells. We also performed knockdown of the CMR genes in mouse embryonic stem (ES) cells and induced in vitro cardiac differentiation. Three CMR genes, AW551984, 2810405K02Rik and Cd302, modulated the cardiac differentiation of both EC cells and ES cells. Depletion of AW551984 attenuated the expression of early cardiac transcription factor Nkx2.5 without affecting transcript levels of pluripotency and early mesoderm marker genes during ES cell differentiation. Activation of Wnt/b-catenin signaling enhanced the expression of both AW551984 and Nkx2.5 in ES cells during embryoid body formation. Our findings indicate that AW551984 is a novel regulator of cardiomyogenesis from pluripotent embryonic cells, which links Wnt/b-catenin signaling to Nkx2.5 expression.
Authors:
Satoshi Yasuda; Tetsuya Hasegawa; Tetsuji Hosono; Mitsutoshi Satoh; Kei Watanabe; Kageyoshi Ono; Shunichi Shimizu; Takao Hayakawa; Teruhide Yamaguchi; Kazuhiro Suzuki; Yoji Sato
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-10
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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