Document Detail

Autophagy deficiency promotes beta-lactam production in Penicillium chrysogenum.
MedLine Citation:
PMID:  21169429     Owner:  NLM     Status:  MEDLINE    
We have investigated the significance of autophagy in the production of the β-lactam antibiotic penicillin (PEN) by the filamentous fungus Penicillium chrysogenum. In this fungus PEN production is compartmentalized in the cytosol and in peroxisomes. We demonstrate that under PEN-producing conditions significant amounts of cytosolic and peroxisomal proteins are degraded via autophagy. Morphological analysis, based on electron and fluorescence microscopy, revealed that this phenomenon might contribute to progressive deterioration of late subapical cells. We show that deletion of the P. chrysogenum ortholog of Saccharomyces cerevisiae serine-threonine kinase atg1 results in impairment of autophagy. In P. chrysogenum atg1 cells, a distinct delay in cell degeneration is observed relative to wild-type cells. This phenomenon is associated with an increase in the enzyme levels of the PEN biosynthetic pathway and enhanced production levels of this antibacterial compound.
Magdalena Bartoszewska; Jan A K W Kiel; Roel A L Bovenberg; Marten Veenhuis; Ida J van der Klei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-12-17
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-09     Completed Date:  2011-08-22     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1413-22     Citation Subset:  IM    
Molecular Cell Biology, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands.
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MeSH Terms
Autophagy* / genetics
Cytosol / metabolism
Fungal Proteins / genetics,  metabolism
Genes, Fungal
Magnetic Resonance Spectroscopy
Microscopy, Electron
Microscopy, Fluorescence
Penicillins / biosynthesis*,  metabolism
Penicillium chrysogenum / enzymology,  genetics,  metabolism,  physiology*
Peroxisomes / metabolism
Polymerase Chain Reaction
Protein-Serine-Threonine Kinases / genetics*,  metabolism
Saccharomyces cerevisiae / genetics
Sequence Analysis, DNA
Sequence Deletion
Reg. No./Substance:
0/Fungal Proteins; 0/Penicillins; EC Kinases

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