| AUTOPHAGY DEFICIENCY PROMOTES {beta}-LACTAM PRODUCTION IN PENICILLIUM CHRYSOGENUM. | |
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MedLine Citation:
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PMID: 21169429 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have investigated the significance of autophagy in the production of the β-lactam antibiotic penicillin (PEN) by the filamentous fungus Penicillium chrysogenum. In this fungus PEN production is compartmentalized in the cytosol and in peroxisomes. We demonstrate that at PEN producing conditions significant amounts of cytosolic and peroxisomal proteins are degraded via autophagy. Morphological analysis, using electron and fluorescence microscopy, revealed that this phenomenon might contribute to progressive deterioration of late sub-apical cells. We show that deletion of the P. chrysogenum ortholog of yeast serine-threonine kinase atg1 results in impairment of autophagy. In P. chrysogenum atg1 cells, a distinct delay in cell degeneration is observed relative to wild type cells. This phenomenon is associated with an increase in the enzyme levels of the PEN biosynthetic pathway and enhanced production levels of this antibacterial compound. |
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Authors:
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Magdalena Bartoszewska; J A K W Kiel; Roel A L Bovenberg; Marten Veenhuis; Ida J van der Klei |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-12-17 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Molecular Cell Biology, Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, P.O. Box 14, 9750 AA Haren, The Netherlands; DSM Biotechnology Center (624-0270), P.O. Box 425, 2600 AK, Delft, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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