Document Detail


ATR regulates a G2-phase cell-cycle checkpoint in Arabidopsis thaliana.
MedLine Citation:
PMID:  15075397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ataxia telangiectasia-mutated and Rad3-related (ATR) plays a central role in cell-cycle regulation, transmitting DNA damage signals to downstream effectors of cell-cycle progression. In animals, ATR is an essential gene. Here, we find that Arabidopsis (Arabidopsis thaliana) atr-/- mutants were viable, fertile, and phenotypically wild-type in the absence of exogenous DNA damaging agents but exhibit altered expression of AtRNR1 (ribonucleotide reductase large subunit) and alteration of some damage-induced cell-cycle checkpoints. atr mutants were hypersensitive to hydroxyurea (HU), aphidicolin, and UV-B light but only mildly sensitive to gamma-radiation. G2 arrest was observed in response to gamma-irradiation in both wild-type and atr plants, albeit with slightly different kinetics, suggesting that ATR plays a secondary role in response to double-strand breaks. G2 arrest also was observed in wild-type plants in response to aphidicolin but was defective in atr mutants, resulting in compaction of nuclei and subsequent cell death. By contrast, HU-treated wild-type and atr plants arrested in G1 and showed no obvious signs of cell death. We propose that, in plants, HU invokes a novel checkpoint responsive to low levels of deoxynucleotide triphosphates. These results demonstrate the important role of cell-cycle checkpoints in the ability of plant cells to sense and cope with problems associated with DNA replication.
Authors:
Kevin Culligan; Alain Tissier; Anne Britt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2004-04-09
Journal Detail:
Title:  The Plant cell     Volume:  16     ISSN:  1040-4651     ISO Abbreviation:  Plant Cell     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-14     Completed Date:  2004-07-19     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1091-104     Citation Subset:  IM; S    
Affiliation:
Section of Plant Biology, University of California, Davis, California 95616, USA. kmculligan@ucdavis.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AB040133;  AF092841
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MeSH Terms
Descriptor/Qualifier:
Aphidicolin / pharmacology
Arabidopsis / drug effects,  enzymology,  genetics*,  radiation effects
Arabidopsis Proteins / genetics
Cell Cycle
Cell Cycle Proteins / genetics*
DNA Replication / drug effects,  radiation effects
Gene Deletion
Hydroxyurea / pharmacology
Molecular Sequence Data
Phenotype
Protein-Serine-Threonine Kinases / genetics*
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Cell Cycle Proteins; 127-07-1/Hydroxyurea; 38966-21-1/Aphidicolin; EC 2.7.1.-/ATR protein, Arabidopsis; EC 2.7.11.1/Protein-Serine-Threonine Kinases
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