Na(+)/K(+)-ATPase expression changes in the rabbit lacrimal glands during pregnancy. | |
MedLine Citation:
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PMID: 23009595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the expressional changes of Na(+)/K(+)-ATPase subunits in the lacrimal glands (LG) of term pregnant rabbits. METHODS: LG were obtained from term pregnant rabbits and age-matched female control rabbits for laser capture microdissection (LCM), real time RT-PCR, western blot, and immunofluorescence. The mRNA and proteins of α1, α2, β1, β2, and β3 subunits of Na(+)/K(+)-ATPase were detected and quantified. RESULTS: Although only the mRNA for β3 from whole LG of pregnant rabbits was significantly different from that of normal controls, many mRNA levels for α1, α2, β1, β2, and β3 from acini and epithelial cells from various duct segments that were collected by LCM were significantly different from those of normal control rabbits. Western blots demonstrated that the expressions of all three β subunits were significantly higher in pregnant rabbits, while both α subunits remained unchanged during pregnancy. Interestingly, immunofluorescence results showed that the distribution patterns of all Na(+)/K(+)-ATPase subunits during pregnancy were similar to those of the control rabbits. CONCLUSIONS: Changes were found in mRNA and protein expressions of Na(+)/K(+)-ATPase subunits in LG from term pregnant rabbits and these changes suggest a role in the pregnancy-related LG secretion changes and dry eye symptoms observed in these animals. |
Authors:
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Jianyan Huang; Michael Lu; Chuanqing Ding |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural Date: 2012-09-25 |
Journal Detail:
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Title: Current eye research Volume: 38 ISSN: 1460-2202 ISO Abbreviation: Curr. Eye Res. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-01-07 Completed Date: 2013-06-04 Revised Date: 2014-01-09 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 18-26 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Disease Models, Animal Dry Eye Syndromes / genetics*, metabolism Epithelial Cells / metabolism, pathology Female Gene Expression Regulation* Immunohistochemistry Lacrimal Apparatus / metabolism*, pathology Laser Capture Microdissection Pregnancy Pregnancy, Animal* RNA, Messenger / genetics* Rabbits Reverse Transcriptase Polymerase Chain Reaction Sodium-Potassium-Exchanging ATPase / biosynthesis, genetics* |
Grant Support | |
ID/Acronym/Agency:
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EY017731/EY/NEI NIH HHS; EY03040/EY/NEI NIH HHS; R01 EY017731/EY/NEI NIH HHS |
Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
Comments/Corrections |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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