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An ATP synthase harboring an atypical γ-subunit is involved in ATP synthesis in tomato fruit chromoplasts.
MedLine Citation:
PMID:  23302027     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chromoplasts are non-photosynthetic plastids specialized in the synthesis and accumulation of carotenoids. During fruit ripening, chloroplasts differentiate into photosynthetically inactive chromoplasts in a process characterized by the degradation of the thylakoid membranes and the active synthesis and accumulation of carotenoids. This transition renders chromoplasts unable to photochemically synthesize ATP and therefore these organelles need to obtain the ATP required for anabolic processes through alternative sources. It is widely accepted that the ATP used for biosynthetic processes in non-photosynthetic plastids is imported from the cytosol or obtained through glycolysis. In this work we show however that isolated tomato fruit chromoplasts are able to synthesize ATP de novo through a respiratory pathway using NADPH as electron donor. We also report the involvement of a plastidial ATP synthase harboring an atypical γ-subunit induced during ripening which lacks the regulatory dithiol domain present in plant and algae chloroplast γ-subunits. Silencing of this atypical γ-subunit during fruit ripening impairs the capacity of isolated chromoplast to synthesize ATP de novo. We propose that the replacement of the γ-subunit present in tomato leaf and green fruit chloroplasts by the atypical γ-subunit lacking the dithiol domain during fruit ripening reflects evolutionary changes which allow the operation of chromoplast ATP synthase under the particular physiological conditions found in this organelle. © 2013 The Authors. The Plant Journal © 2013 Blackwell Publishing Ltd.
Authors:
Irini Pateraki; Marta Renato; Joaquín Azcón-Bieto; Albert Boronat
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors. The Plant Journal © 2013 Blackwell Publishing Ltd.
Affiliation:
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 643, 08028, Barcelona, Spain; Centre de Recerca en Agrigenòmica (CRAG), Consorci CSIC-IRTA-UAB-UB, Campus Universitat Autònoma de Barcelona, Bellaterra - Cerdanyola del Vallès, 08193, Barcelona, Spain.
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