Document Detail


ATP promotes extracellular matrix biosynthesis of intervertebral disc cells.
MedLine Citation:
PMID:  25407524     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We have recently found a high accumulation of extracellular adenosine triphosphate (ATP) in the center of healthy porcine intervertebral discs (IVD). Since ATP is a powerful extracellular signaling molecule, extracellular ATP accumulation might regulate biological activities in the IVD. The objective of this study was therefore to investigate the effects of extracellular ATP on the extracellular matrix (ECM) biosynthesis of porcine IVD cells isolated from two distinct anatomical regions: the annulus fibrosus (AF) and nucleus pulposus (NP). ATP treatment significantly promotes ECM deposition and corresponding gene expression (aggrecan and type II collagen) by both cell types in three-dimensional agarose culture. A significant increase in ECM accumulation has been found in AF cells at a lower ATP treatment level (20 μM) compared with NP cells (100 μM), indicating that AF cells are more sensitive to extracellular ATP than NP cells. NP cells also exhibit higher ECM accumulation and intracellular ATP than AF cells under control and treatment conditions, suggesting that NP cells are intrinsically more metabolically active. Moreover, ATP treatment also augments the intracellular ATP level in NP and AF cells. Our findings suggest that extracellular ATP not only promotes ECM biosynthesis via a molecular pathway, but also increases energy supply to fuel that process.
Authors:
Silvia Gonzales; Chong Wang; Howard Levene; Herman S Cheung; Chun-Yuh Charles Huang
Related Documents :
5387974 - Biochemical and cytological heterogeneity of the differentiating cells of the cellular ...
25447674 - Indole-3-carbinol inhibits tumorigenicity of hepatocellular carcinoma cells via suppres...
112094 - Aggregation of sponge cells. function of a lectin in its homologous biological system.
20171364 - Expression of the inflammatory marker cyclooxygenase-2 in dental pulp cells cultured wi...
22465804 - Eriodictyol-7-o-glucoside, a novel nrf2 activator, confers protection against cisplatin...
11352674 - Responses of coxsackievirus b4-specific t-cell lines to 2c protein-characterization of ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-19
Journal Detail:
Title:  Cell and tissue research     Volume:  -     ISSN:  1432-0878     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-19     Completed Date:  -     Revised Date:  2014-11-20    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Spontaneous repair of partial thickness linear cartilage injuries in immature rats.
Next Document:  The Stability of G6PD Is Affected by Mutations with Different Clinical Phenotypes.