| ATP-induced currents carried through P2X7 receptor in rat myometrial cells. | |
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MedLine Citation:
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PMID: 22814097 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objective: The functional significance of purinergic P2 receptors in the myometrium is unclear. We previously reported the ATP-induced ionic currents in rat myometrial cells, causing uterine contractility. The aim of this study is to determine the main P2X receptors that carry the adenosine triphosphate (ATP)-induced currents. Study Design: We cloned predominantly expressed P2X7 receptors from rat myometrium and transfected into cultured COS-7 cells. Reconstructed P2X7 receptor currents were characterized using the whole-cell patch clamp method. Results: Extracellular ATP induced currents through P2X7 receptors with effective concentration (EC(50)) of 155 μmol/L, without desensitization. The myometrial P2X7 receptor was permeable to multiple monovalent cations with conductances ranked as K(+)>Cs(+)>Li(+)>Na(+). It was activated by P2X receptor agonists, with effectiveness ranked as 2',3'-O-(4-benzoylbenzoyl)-ATP (Bz-ATP)>ATP>αβ-methylene ATP (αβ-MeATP)>2-methylthio ATP (2-MeSATP)>uridine triphosphate (UTP)>guanosine triphosphate (GTP)>adenosine diphosphate (ADP). These currents were blocked by selective P2X7 receptor antagonists and extracellular Mg(2+). Conclusion: P2X7 receptors carry ATP-induced currents in rat myometrial cells. |
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Authors:
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Hiroshi Miyoshi; Kaoru Yamaoka; Satoshi Urabe; Yoshiki Kudo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-18 |
Journal Detail:
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Title: Reproductive sciences (Thousand Oaks, Calif.) Volume: - ISSN: 1933-7205 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101291249 Medline TA: Reprod Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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