Document Detail

ATP depletion increases tyrosine phosphorylation of beta-catenin and plakoglobin in renal tubular cells.
MedLine Citation:
PMID:  10541288     Owner:  NLM     Status:  MEDLINE    
This study examines the hypothesis that the loss of integrity of the junctional complex induced by ATP depletion is related to alterations in tyrosine phosphorylation of the adherens junction proteins beta-catenin and plakoglobin. ATP depletion of cultured mouse proximal tubular (MPT) cells induces a marked increase in tyrosine phosphorylation of both beta-catenin and plakoglobin. The tyrosine phosphatase inhibitor vanadate has the same effect in ATP-replete (control) monolayers, whereas genistein, a tyrosine kinase inhibitor, reduces phosphorylation of both proteins in ATP-replete monolayers and prevents the hyperphosphorylation of these proteins with ATP depletion. This study also demonstrates that the fall in the transepithelial resistance of MPT monolayers induced by ATP depletion can be reproduced by treatment of ATP-replete monolayers with vanadate, whereas genistein substantially ameliorates the fall in transepithelial resistance induced by ATP depletion. Also, using immunofluorescence microscopy it was demonstrated that ATP depletion results in a marked diminution of E-cadherin staining in the basolateral membrane of MPT cells. Vanadate mimics this effect of ATP depletion, whereas genistein ameliorates the reduction in the intensity of E-cadherin staining induced by ATP depletion. Because it is has been well established that hyperphosphorylation of the catenins leads to dissociation of the adherens junction and to dysfunction of the junctional complex, it is proposed that the increase in tyrosine phosphorylation of catenins observed in MPT cells during ATP depletion contributes to the loss of function of the junctional complex associated with sublethal injury.
J H Schwartz; T Shih; S A Menza; W Lieberthal
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  10     ISSN:  1046-6673     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-18     Completed Date:  1999-11-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2297-305     Citation Subset:  IM    
Evans Department of Clinical Research, Boston Medical Center, Massachusetts 02118, USA.
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MeSH Terms
Adenosine Triphosphate / physiology*
Cadherins / analysis
Cells, Cultured
Cytoskeletal Proteins / metabolism*
Fluorescent Antibody Technique
Kidney Tubules, Proximal / metabolism*
Mice, Inbred C57BL
Tyrosine / metabolism*
beta Catenin
gamma Catenin
Grant Support
Reg. No./Substance:
0/Cadherins; 0/Catnb protein, mouse; 0/Cytoskeletal Proteins; 0/Desmoplakins; 0/Trans-Activators; 0/beta Catenin; 0/gamma Catenin; 55520-40-6/Tyrosine; 56-65-5/Adenosine Triphosphate

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