Document Detail

ATP-dependent DNA aggregation is a novel function of rat serum albumin.
MedLine Citation:
PMID:  1898009     Owner:  NLM     Status:  MEDLINE    
An ATP-dependent DNA aggregating activity was purified from rat liver by DEAE-cellulose, phosphocellulose, and novobiocin-Sepharose column chromatography. The protein aggregated superhelical, relaxed, single-, or double-stranded DNA in a divalent cation- and ATP-dependent reaction. The DNA aggregating activity was detected by retardation of a DNA-protein complex at the origin on a 1% agarose gel. The protein appeared to exist in solution as a monomer of molecular weight 66,000, and had no DNA polymerase, topoisomerase, recombinase, or ligase activity. The DNA aggregating activity was inhibited by 10 mM nalidixic acid or 1 mM novobiocin but not by 20 mM N-ethylmaleimide or camptothecin. Adenylyl(beta,gamma-methylene)-diphosphonate, adenylyl-imidodiphosphate, or adenosine-5'-O(3-thiotriphosphate) did not substitute for ATP whereas CTP, dTTP, or the ATP analog adenylyl(alpha,beta-methylene)-diphosphonate could replace ATP. The aggregated DNA was only partially dissociated by restriction endonuclease digestion but was completely dissociated by deproteinization with SDS, proteinase K, or chloroform/octanol extraction. On the basis of the molecular weight, thermostability, antigenic property, and amino acid sequence homology in the first 12 positions, we conclude that the rat liver protein is serum albumin and that the ATP-dependent DNA aggregation is a novel function of rat serum albumin.
O Nyormoi; R E Moses
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  287     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-10-24     Completed Date:  1991-10-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  367-71     Citation Subset:  IM    
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Adenosine Triphosphate / pharmacology*
Cations, Divalent
DNA / drug effects,  metabolism*
DNA, Circular / drug effects,  metabolism
DNA, Single-Stranded / drug effects,  metabolism
DNA, Superhelical / drug effects,  metabolism
DNA, Viral / drug effects,  metabolism
Drug Stability
Hot Temperature
Liver / chemistry*
Macromolecular Substances
Molecular Weight
Nalidixic Acid / pharmacology
Novobiocin / pharmacology
Rats, Inbred Strains
Sequence Homology, Nucleic Acid
Serum Albumin / chemistry,  isolation & purification,  pharmacology*
Grant Support
Reg. No./Substance:
0/Cations, Divalent; 0/DNA, Circular; 0/DNA, Single-Stranded; 0/DNA, Superhelical; 0/DNA, Viral; 0/Macromolecular Substances; 0/Serum Albumin; 303-81-1/Novobiocin; 389-08-2/Nalidixic Acid; 56-65-5/Adenosine Triphosphate; 9007-49-2/DNA

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