| ATP-dependent DNA aggregation is a novel function of rat serum albumin. | |
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MedLine Citation:
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PMID: 1898009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An ATP-dependent DNA aggregating activity was purified from rat liver by DEAE-cellulose, phosphocellulose, and novobiocin-Sepharose column chromatography. The protein aggregated superhelical, relaxed, single-, or double-stranded DNA in a divalent cation- and ATP-dependent reaction. The DNA aggregating activity was detected by retardation of a DNA-protein complex at the origin on a 1% agarose gel. The protein appeared to exist in solution as a monomer of molecular weight 66,000, and had no DNA polymerase, topoisomerase, recombinase, or ligase activity. The DNA aggregating activity was inhibited by 10 mM nalidixic acid or 1 mM novobiocin but not by 20 mM N-ethylmaleimide or camptothecin. Adenylyl(beta,gamma-methylene)-diphosphonate, adenylyl-imidodiphosphate, or adenosine-5'-O(3-thiotriphosphate) did not substitute for ATP whereas CTP, dTTP, or the ATP analog adenylyl(alpha,beta-methylene)-diphosphonate could replace ATP. The aggregated DNA was only partially dissociated by restriction endonuclease digestion but was completely dissociated by deproteinization with SDS, proteinase K, or chloroform/octanol extraction. On the basis of the molecular weight, thermostability, antigenic property, and amino acid sequence homology in the first 12 positions, we conclude that the rat liver protein is serum albumin and that the ATP-dependent DNA aggregation is a novel function of rat serum albumin. |
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Authors:
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O Nyormoi; R E Moses |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 287 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1991 Jun |
Date Detail:
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Created Date: 1991-10-24 Completed Date: 1991-10-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 367-71 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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pharmacology* Animals Cations, Divalent DNA / drug effects, metabolism* DNA, Circular / drug effects, metabolism DNA, Single-Stranded / drug effects, metabolism DNA, Superhelical / drug effects, metabolism DNA, Viral / drug effects, metabolism Drug Stability Hot Temperature Liver / chemistry* Macromolecular Substances Molecular Weight Nalidixic Acid / pharmacology Novobiocin / pharmacology Rats Rats, Inbred Strains Sequence Homology, Nucleic Acid Serum Albumin / chemistry, isolation & purification, pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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G13286//PHS HHS; GM24711/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cations, Divalent; 0/DNA, Circular; 0/DNA, Single-Stranded; 0/DNA, Superhelical; 0/DNA, Viral; 0/Macromolecular Substances; 0/Serum Albumin; 303-81-1/Novobiocin; 389-08-2/Nalidixic Acid; 56-65-5/Adenosine Triphosphate; 9007-49-2/DNA |
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