| ATP- and coenzyme A-dependent fatty acid incorporation into proteins of cell-free extracts from mouse tissues. | |
| | |
MedLine Citation:
|
PMID: 3941109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The incorporation of tritiated fatty acids into proteins has been studied in cell-free extracts from mouse tissues. Incubation of heart extracts with [3H]tetradecanoic or [3H]palmitic acid in the presence of ATP and CoA resulted in the time-dependent and selective labeling of proteins (Mr = 60,000, 47,000, 42,000, 31,000, 16,000, and 13,000) which could be detected after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. Two polypeptides (Mr = 47,000 and 42,000) reached a maximum in fatty acid incorporation very rapidly and were mainly localized in the membrane subcellular fractions of the extract. These proteins underwent transient labeling with [3H] tetradecanoyl-CoA, the maximum incorporation being obtained within 1 min. The fatty acid-labeled proteins from tissue extracts had the same properties as other proteins known to be acylated in intact cells, i.e. the acyl moiety was resistant to delipidation with organic solvents but could be hydrolyzed by treatment with neutral hydroxylamine. Screening of different tissues showed that extracts from liver and kidney also catalyze the ATP- and CoA-dependent formation of a similar group of fatty acid-acylated proteins. The results provide evidence for a group of proteins in mammalian tissues which selectively incorporate fatty acids in vitro and should be of value for further studies on the biosynthesis of acylated proteins. |
| | |
Authors:
|
D Riendeau; D Guertin |
Related Documents
:
|
7696329 - Subcellular localisation and induction of nadh-sensitive acetyl-coa hydrolase and propi... 8930759 - Development of a simple liquid chromatographic method for the separation of mixtures of... 5965339 - The fate of acetyl groups derived from glucose in the isolated perfused goat udder. 16492739 - Structure-based inhibitor design of accd5, an essential acyl-coa carboxylase carboxyltr... 16996019 - A homogeneous scintillation proximity assay for acetyl coenzyme a carboxylase coupled t... 8944819 - Increased cardiac fatty acid uptake with dobutamine infusion in swine is accompanied by... 16475819 - Transmembrane helices 3 and 4 are involved in substrate recognition by the na+/dicarbox... 2552569 - Stimulatory effect of short-chain fatty acids on epithelial cell proliferation of isola... 2227729 - Evaluation of a commercial radioimmunoassay kit for the detection of lysergide (lsd) in... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 261 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1986 Jan |
Date Detail:
|
Created Date: 1986-02-14 Completed Date: 1986-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 976-81 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
metabolism* Animals Cell-Free System Coenzyme A / metabolism* Electrophoresis, Polyacrylamide Gel Fatty Acids / metabolism* Fluorometry Hydroxylamine Hydroxylamines / pharmacology Mercaptoethanol / pharmacology Mice Molecular Weight Myocardium / metabolism Palmitic Acid Palmitic Acids / metabolism Proteins / metabolism* Subcellular Fractions / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Hydroxylamines; 0/Palmitic Acids; 0/Proteins; 56-65-5/Adenosine Triphosphate; 57-10-3/Palmitic Acid; 60-24-2/Mercaptoethanol; 7803-49-8/Hydroxylamine; 85-61-0/Coenzyme A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Purification and properties of an (ADP-ribose)n glycohydrolase from guinea pig liver nuclei.
Next Document: Sternocostoclavicular hyperostosis. A report of nineteen cases, with special reference to etiology a...