Document Detail

ATP breakdown products in human skeletal muscle during prolonged exercise to exhaustion.
MedLine Citation:
PMID:  3427883     Owner:  NLM     Status:  MEDLINE    
To study changes in muscle energy state during prolonged exercise, especially in relation to fatigue, muscle biopsies were obtained from seven healthy males working until exhaustion on a cycle ergometer at 68% (63-74%) of their maximal oxygen uptake. Biopsies were taken at rest, after 15 and 45 min of exercise and at exhaustion, and analysed for ATP, ADP, AMP, inosine monophosphate (IMP) and hypoxanthine content by high performance liquid chromatography (HPLC), and for creatine phosphate (CP), lactate and glycogen by enzymatic fluorometric techniques. Glycogen content at exhaustion was approximately 30% of the pre-exercise level. The CP content decreased steeply during the first 15 min of exercise (P less than 0.01) and continued to decrease during the rest of the exercise period (P less than 0.05). Pronounced increases in contents of IMP (64% P less than 0.001) and hypoxanthine (69%, P less than 0.05) were found when exhaustion was approaching. Furthermore, energy charge [EC; (ATP + 0.5 ADP)/(ATP + ADP + AMP)] was decreased at exhaustion (P less than 0.05). The increases in IMP and hypoxanthine which occurred when exhaustion was approaching during prolonged submaximal exercise together with the decrease in EC during this phase of exercise suggest a failure of the exercising skeletal muscle to regenerate ATP at exhaustion.
B Norman; A Sollevi; L Kaijser; E Jansson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical physiology (Oxford, England)     Volume:  7     ISSN:  0144-5979     ISO Abbreviation:  Clin Physiol     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-02-29     Completed Date:  1988-02-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8309768     Medline TA:  Clin Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  503-10     Citation Subset:  IM; S    
Department of Clinical Physiology, Karolinska Hospital, Stockholm, Sweden.
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MeSH Terms
Adenosine Diphosphate / metabolism
Adenosine Monophosphate / metabolism
Adenosine Triphosphate / metabolism*
Fatigue / metabolism*
Glycogen / metabolism
Hypoxanthines / metabolism
Inosine Monophosphate / metabolism
Muscles / metabolism*
Phosphocreatine / metabolism
Physical Exertion*
Reg. No./Substance:
0/Hypoxanthines; 131-99-7/Inosine Monophosphate; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 61-19-8/Adenosine Monophosphate; 67-07-2/Phosphocreatine; 68-94-0/Hypoxanthine; 9005-79-2/Glycogen

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