Document Detail


ATP Regulates Sodium Channel Kinetics in Pancreatic Islet Beta Cells.
MedLine Citation:
PMID:  23296347     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Pancreatic beta cells act as glucose sensors, in which intracellular ATP ([ATP](i)) are altered with glucose concentration change. The characterization of voltage-gated sodium channels under different [ATP](i) remains unclear. Here, we demonstrated that increasing [ATP](i) within a certain range of concentrations (2-8 mM) significantly enhanced the voltage-gated sodium channel currents, compared with 2 mM cytosolic ATP. This enhancement was attenuated by even high intracellular ATP (12 mM). Furthermore, elevated ATP modulated the sodium channel kinetics in a dose-dependent manner. Increased [ATP](i) shifted both the current-voltage curve and the voltage-dependent inactivation curve of sodium channel to the right. Finally, the sodium channel recovery from inactivation was significantly faster when the intracellular ATP level was increased, especially in 8 mM [ATP](i), which is an attainable concentration by the high glucose stimulation. In summary, our data suggested that elevated cytosolic ATP enhanced the activity of Na(+) channels, which may play essential roles in modulating β cell excitability and insulin release when blood glucose concentration increases.
Authors:
Na Zou; Xiao Wu; Yan-Yan Jin; Meng-Zao He; Xin-Xin Wang; Li-Da Su; Marjan Rupnik; Zhen-Yong Wu; Li Liang; Ying Shen
Related Documents :
20083117 - Calcium sensitivity, force frequency relationship and cardiac troponin i: critical role...
1880817 - The human atrial trabecula: effects of calcium and temperature.
8989647 - Molecular basis of depression of ca2+ sensitivity of tension by acid ph in cardiac musc...
2110967 - Mechanical transients of single toad stomach smooth muscle cells. effects of lowering t...
9545267 - Dgnac1, a gonad-specific amiloride-sensitive na+ channel.
1491427 - Anion-dependent transport of thallous ions through human erythrocyte membrane.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-8
Journal Detail:
Title:  The Journal of membrane biology     Volume:  -     ISSN:  1432-1424     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Neurobiology, Key Laboratory of Medical Neurobiology of Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Anterosuperior rotator cuff tears : Rupture configurations and results after arthroscopic therapy - ...
Next Document:  SENSITIVITY ANALYSIS FOR OSCILLATING DYNAMICAL SYSTEMS.