| ATP production rate via creatine kinase or ATP synthase in vivo: a novel superfast magnetization saturation transfer method. | |
| | |
MedLine Citation:
|
PMID: 21293002 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
RATIONALE: ³¹P magnetization saturation transfer (MST) experiment is the most widely used method to study ATP metabolism kinetics. However, its lengthy data acquisition time greatly limits the wide biomedical applications in vivo, especially for studies requiring high spatial and temporal resolutions. OBJECTIVE: We aimed to develop a novel superfast MST method that can accurately quantify ATP production rate constants (k(f)) through creatine kinase (CK) or ATP synthase (ATPase) with 2 spectra. METHODS AND RESULTS: The T₁(nom) (T₁ nominal) method uses a correction factor to compensate the partially relaxed MST experiments, thus allowing measurement of enzyme kinetics with an arbitrary repetition time and flip angle, which consequently reduces the data acquisition time of a transmurally differentiated CK k(f) measurement by 91% as compared with the conventional method with spatial localization. The novel T₁(nom) method is validated theoretically with numeric simulation, and further verified with in vivo swine hearts, as well as CK and ATPase activities in rat brain at 9.4 Tesla. Importantly, the in vivo data from swine hearts demonstrate, for the first time, that within an observation window of 30 minutes, the inhibition of CK activity by iodoacetamide does not limit left ventricular chamber contractile function. CONCLUSIONS: A novel MST method for superfast examination of enzyme kinetics in vivo has been developed and verified theoretically and experimentally. In the in vivo normal heart, redundant multiple supporting systems of myocardial ATP production, transportation, and utilization exist, such that inhibition of one mechanism does not impair the normal left ventricular contractile performance. |
| | |
Authors:
|
Qiang Xiong; Fei Du; Xiaohong Zhu; Pengyuan Zhang; Piradeep Suntharalingam; Joseph Ippolito; Forum D Kamdar; Wei Chen; Jianyi Zhang |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-03 |
Journal Detail:
|
Title: Circulation research Volume: 108 ISSN: 1524-4571 ISO Abbreviation: Circ. Res. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-03-18 Completed Date: 2011-05-23 Revised Date: 2013-03-25 |
Medline Journal Info:
|
Nlm Unique ID: 0047103 Medline TA: Circ Res Country: United States |
Other Details:
|
Languages: eng Pagination: 653-63 Citation Subset: IM |
Affiliation:
|
Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
biosynthesis* Animals Creatine Kinase / antagonists & inhibitors, metabolism* Energy Metabolism Female Hemodynamics Kinetics Magnetic Resonance Imaging Magnetic Resonance Spectroscopy / methods* Male Models, Biological Myocardium / metabolism Phosphorus Isotopes Proton-Translocating ATPases / metabolism* Rats Rats, Sprague-Dawley Swine Time Factors |
| Grant Support | |
ID/Acronym/Agency:
|
HL 100407/HL/NHLBI NIH HHS; HL 67828/HL/NHLBI NIH HHS; HL 95077/HL/NHLBI NIH HHS; HL50470/HL/NHLBI NIH HHS; NS041262/NS/NINDS NIH HHS; NS057560/NS/NINDS NIH HHS; NS070839/NS/NINDS NIH HHS; P30 NS057091/NS/NINDS NIH HHS; P41 RR008079/RR/NCRR NIH HHS; R01 HL050470-08/HL/NHLBI NIH HHS; R01 HL067828-09/HL/NHLBI NIH HHS; R01 HL095077-03/HL/NHLBI NIH HHS; U01 HL100407/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Phosphorus Isotopes; 56-65-5/Adenosine Triphosphate; EC 2.7.3.2/Creatine Kinase; EC 3.6.3.14/Proton-Translocating ATPases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human Resistin Stimulates Hepatic Overproduction of Atherogenic ApoB-Containing Lipoprotein Particle...
Next Document: Docking Protein Gab1 Is an Essential Component of Postnatal Angiogenesis After Ischemia via HGF/c-Me...