| ATP-loaded liposomes for targeted treatment in models of myocardial ischemia. | |
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MedLine Citation:
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PMID: 20072894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ATP cannot be effectively delivered to most tissues including the ischemic myocardium without protection from degradation by plasma endonucleotidases. However, it has been established that ATP can be delivered to various tissues by its encapsulation within liposomal preparations. We describe here, the materials needed and methods used to optimize the encapsulation of ATP in liposomes, enhance their effectiveness by increasing their circulation time and target injured myocardial cells with liposomal surface anti-myosin antibody. Additionally, we outline methods for ex vivo studies of these ATP liposomal preparations in an isolated ischemic rat heart model and for in vivo studies of rabbits with an induced myocardial infarction. The expectation is that these methods will provide a basis for continued studies of effective ways to deliver energy substrates to the ischemic myocardium. |
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Authors:
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Tatyana S Levchenko; William C Hartner; Daya D Verma; Eugene A Bernstein; Vladimir P Torchilin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 605 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 361-75 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Sciences, Bouve College of Health Sciences, Northeastern University, Boston, MA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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administration & dosage*,
therapeutic use Animals Antibodies / immunology* Heart / drug effects, physiopathology Liposomes / chemistry, immunology*, pharmacokinetics Myocardial Ischemia / drug therapy* Myocardium / pathology Myosins / immunology* Rabbits Rats |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Liposomes; 56-65-5/Adenosine Triphosphate; EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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