| AT1 angiotensin II receptor mediates intracellular calcium mobilization in normal and cancerous breast cells in primary culture. | |
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MedLine Citation:
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PMID: 12127057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Angiotensin II (Ang II) increases intracellular calcium concentration ([Ca2+]i) in both normal and cancerous human breast cells in primary culture. Maximal [Ca2+]i increase is obtained using 100nM Ang II in both cell types; in cancerous breast cells, [Ca2+]i increase (delta[Ca2+]i) is 135+/-10nM, while in normal breast cells it reaches 65+/-5 nM (P<0.0001). In both cell types, Ang II evokes a Ca2+ transient peak mediated by thapsigargin (TG) sensitive stores; neither Ca2+ entry through L-type membrane channels or capacitative Ca2+ entry are involved. Type I Ang II receptor subtype (AT1) mediates Ang II-dependent [Ca2+]i increase, since losartan, an AT1 inhibitor, blunted [Ca2+]i increase induced by Ang II in a dose-dependent manner, while CGP 4221A, an AT2 inhibitor, does not. Phospholipase C (PLC) is involved in this signaling mechanism, as U73122, a PLC inhibitor, decreases Ang II-dependent [Ca2+]i transient peak in a dose-dependent mode.Thus, the present study provides new information about Ca2+ signaling pathways mediated through AT1 in breast cells in which no data were yet available. |
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Authors:
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S Greco; M G Elia; A Muscella; C Storelli; S Marsigliante |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell calcium Volume: 32 ISSN: 0143-4160 ISO Abbreviation: Cell Calcium Publication Date: 2002 Jul |
Date Detail:
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Created Date: 2002-07-19 Completed Date: 2003-02-14 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8006226 Medline TA: Cell Calcium Country: Scotland |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Affiliation:
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Laboratorio di Fisiologia Generale, Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università di Lecce, Via Provinciale per Monteroni, 73100 Lecce, Italy. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiotensin II
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pharmacology Breast Neoplasms* Calcium / metabolism* Calcium Signaling / drug effects, physiology Epithelial Cells / cytology, drug effects, metabolism Female Gene Expression Humans Receptor, Angiotensin, Type 1 Receptors, Angiotensin / genetics, metabolism* Tumor Cells, Cultured Type C Phospholipases / metabolism Vasoconstrictor Agents / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Receptor, Angiotensin, Type 1; 0/Receptors, Angiotensin; 0/Vasoconstrictor Agents; 11128-99-7/Angiotensin II; 7440-70-2/Calcium; EC 3.1.4.-/Type C Phospholipases |
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