Document Detail

Acid-sensing ion channel 3 deficiency increases inflammation but decreases pain behavior in murine arthritis.
MedLine Citation:
PMID:  23335302     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Through its location on nociceptors, acid-sensing ion channel 3 (ASIC-3) is activated by decreases in pH and plays a significant role in musculoskeletal pain. We recently showed that decreases in pH activate ASIC-3 located on fibroblast-like synoviocytes (FLS), which are key cells in the inflammatory process. The purpose of this study was to test whether ASIC-3-deficient mice with arthritis have altered inflammation and pain relative to controls.
METHODS: Collagen antibody-induced arthritis (CAIA) was generated by injection of an anti-type II collagen antibody cocktail. Inflammation and pain parameters in ASIC-3(-/-) and ASIC-3(+/+) mice were assessed. Disease severity was assessed by determining clinical arthritis scores, measuring joint diameters, analyzing joint histology, and assessing synovial gene expression by quantitative polymerase chain reaction analysis. Cell death was assessed with a Live/Dead assay of FLS in response to decreases in pH. Pain behaviors in the mice were measured by examining withdrawal thresholds in the joints and paws and by measuring their physical activity levels.
RESULTS: Surprisingly, ASIC-3(-/-) mice with CAIA demonstrated significantly increased joint inflammation, joint destruction, and expression of interleukin-6 (IL-6), matrix metalloproteinase 3 (MMP-3), and MMP-13 in joint tissue as compared to ASIC-3(+/+) mice. ASIC-3(+/+) FLS showed enhanced cell death when exposed to pH 6.0 in the presence of IL-1β, which was abolished in ASIC-3(-/-) FLS. Despite enhanced disease severity, ASIC-3(-/-) mice did not develop mechanical hypersensitivity of the paw and showed greater levels of physical activity.
CONCLUSION: Our findings are consistent with the hypothesis that ASIC-3 plays a protective role in the inflammatory arthritides by limiting inflammation through enhanced synoviocyte cell death, which reduces disease severity, and through the production of pain, which reduces joint use.
Kathleen A Sluka; Lynn A Rasmussen; Meghan M Edgar; James M O'Donnell; Roxanne Y Walder; Sandra J Kolker; David L Boyle; Gary S Firestein
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  65     ISSN:  1529-0131     ISO Abbreviation:  Arthritis Rheum.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-24     Completed Date:  2013-06-18     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1194-202     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2013 by the American College of Rheumatology.
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MeSH Terms
Acid Sensing Ion Channels / deficiency*
Arthritis, Experimental / complications,  pathology*,  physiopathology
Arthritis, Rheumatoid / complications,  pathology*,  physiopathology
Behavior, Animal
Cell Death
Cell Survival
Gene Expression
Interleukin-6 / genetics,  metabolism
Joints / metabolism,  pathology,  physiopathology
Matrix Metalloproteinases / genetics,  metabolism
Mice, Inbred C57BL
Mice, Knockout
Pain / etiology,  pathology*,  physiopathology
Pain Measurement
Pain Threshold
Severity of Illness Index
Synovitis / etiology,  pathology*,  physiopathology
Grant Support
Reg. No./Substance:
0/Accn3 protein, mouse; 0/Acid Sensing Ion Channels; 0/Interleukin-6; EC 3.4.24.-/Matrix Metalloproteinases

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