Document Detail


ASE extraction method for simultaneous carbon and nitrogen stable isotope analysis in soft tissues of aquatic organisms.
MedLine Citation:
PMID:  19446063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since lipids are depleted in 13C relative to proteins and carbohydrates, variations in lipid composition among species and within individuals significantly influence delta13C and may result in misleading ecological interpretations. Whereas lipid extraction before IRMS analysis constitutes a way of stable isotope result lipid-normalisation, such a procedure was given up because of the un-controlled effects of the methods used (i.e., "Bligh & Dyer", Soxhlet, etc.) on delta15N. The aim of this work was to develop a simple, rapid and efficient lipid extraction method allowing for simultaneous C and N stable isotope analysis in the biological soft tissues of aquatic organisms. The goal was to be free from the lipid influence on delta13C values without interfering with delta15N values. For that purpose, the modern automated pressurized liquid extraction technique ASE (accelerated solvent extraction) was selected. Eel muscles representative of a broad range of fat contents were extracted via ASE by using different semi-polar solvents (100% dichloromethane and 80% n-hexane/20% acetone) and by operating at different temperature (ambient temperature and 100 degrees C) and pressure (750 and 1900 psi) conditions. The results were discussed in terms of lipid extraction efficiency as well as delta13C and delta15N variability.
Authors:
Nathalie Bodin; Hélène Budzinski; Karyn Le Ménach; Nathalie Tapie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-05
Journal Detail:
Title:  Analytica chimica acta     Volume:  643     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-07-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  54-60     Citation Subset:  IM    
Affiliation:
ISM/LPTC, UMR 5255 CNRS, Université de Bordeaux 1, 351 Cours de la Libération, 33405 Talence, France.
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MeSH Terms
Descriptor/Qualifier:
Anguilla*
Animals
Carbon Isotopes / analysis*
Chemical Fractionation / methods*
Lipids / analysis*
Muscle, Skeletal / chemistry*
Nitrogen Isotopes / analysis*
Pressure
Reproducibility of Results
Solvents / chemistry
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Lipids; 0/Nitrogen Isotopes; 0/Solvents

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