Document Detail


APC is a component of an organizing template for cortical microtubule networks.
MedLine Citation:
PMID:  15892196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microtubule network on the basal cortex of polarized epithelial cells consists of non-centrosomal microtubules of mixed polarity. Here, we investigate the proteins that are involved in organizing this network, and we show that end-binding protein 1 (EB1), adenomatous polyposis coli protein (APC) and p150Glued - although considered to be microtubule plus-end-binding proteins - are localized along the entire length of microtubules within the network, and at T-junctions between microtubules. The network shows microtubule behaviours that arise from physical interactions between microtubules, including microtubule plus-end stabilization on the sides of other microtubules, and sliding of microtubule ends along other microtubules. APC also localizes to the basal cortex. Microtubules grew over and paused at APC puncta; an in vitro reconstituted microtubule network overlaid APC puncta; and microtubule network reconstitution was inhibited by function-blocking APC antibodies. Thus, APC is a component of a cortical template that guides microtubule network formation.
Authors:
Amy Reilein; W James Nelson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nature cell biology     Volume:  7     ISSN:  1465-7392     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-12     Completed Date:  2005-06-30     Revised Date:  2012-09-19    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  463-73     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Physiology, Beckman Center for Molecular and Genetic Medicine B121, 279 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5435, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenomatous Polyposis Coli Protein / metabolism*
Animals
Cell Line
Cell Polarity / physiology
Dogs
Microtubule-Associated Proteins / metabolism*
Microtubules / metabolism*,  ultrastructure
Protein Binding
Tubulin / metabolism
Grant Support
ID/Acronym/Agency:
5T32CA09151/CA/NCI NIH HHS; NS 42735/NS/NINDS NIH HHS; R01 GM035527/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Adenomatous Polyposis Coli Protein; 0/EB1 microtubule binding proteins; 0/Microtubule-Associated Proteins; 0/Tubulin; 144198-36-7/dynactin
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