Document Detail


The AP3 adaptor is involved in the transport of membrane proteins to acidocalcisomes of Leishmania.
MedLine Citation:
PMID:  18252798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysosomal function is crucial for the differentiation and infectivity of the parasitic protozoon Leishmania major. To study lysosomal biogenesis, an L. major mutant deficient in the delta subunit of the adaptor protein 3 (AP3 delta) complex was generated. Structure and proteolytic capacity of the lysosomal compartment were apparently unaffected in the AP3-deficient mutant; however, defects were identified in its acidocalcisomes. These are acidic organelles enriched in calcium and phosphorus, conserved from bacteria to eukaryotes, whose function remains enigmatic. The acidocalcisomes of the L. major mutant lacked membrane-bound proton pumps (notably V-H+-PPase), were less acidic than normal acidocalcisomes and devoid of polyphosphate, but contained a soluble pyrophosphatase. The mutant parasites were viable in vitro, but were unable to establish an infection in mice, which indicates a role for AP3 in determining--possibly through an acidocalcisome-related function--the virulence of the parasite. AP3 transport function has been linked previously to lysosome-related organelles such as platelet dense granules, which appear to share several features with acidocalcisomes. Our findings, implicating that AP3 has a role in transport to acidocalcisomes, thus provide further evidence that biogenesis of acidocalcisomes resembles that of lysosome-related organelles, and that both may have conserved origins.
Authors:
Sébastien Besteiro; Daniela Tonn; Laurence Tetley; Graham H Coombs; Jeremy C Mottram
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-05
Journal Detail:
Title:  Journal of cell science     Volume:  121     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-21     Completed Date:  2008-07-09     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  561-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adaptor Protein Complex 3 / genetics,  metabolism*
Animals
Calcium / metabolism
Calcium Signaling / physiology
Leishmania / metabolism*
Lysosomes / metabolism
Membrane Proteins / metabolism*
Organelles / metabolism*
Phosphorus / metabolism
Protein Transport / physiology
Proton Pumps / metabolism
Protozoan Proteins / metabolism
Pyrophosphatases / metabolism
Grant Support
ID/Acronym/Agency:
G0000508//Medical Research Council; //Medical Research Council
Chemical
Reg. No./Substance:
0/Adaptor Protein Complex 3; 0/Membrane Proteins; 0/Proton Pumps; 0/Protozoan Proteins; 27YLU75U4W/Phosphorus; EC 3.6.1.-/Pyrophosphatases; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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