Document Detail

AP-1 and heat shock protein 27 expression in human astrocytomas.
MedLine Citation:
PMID:  11768612     Owner:  NLM     Status:  MEDLINE    
PURPOSE: In previous work we have shown that the expression of heat shock protein 27 (Hsp27) is associated with anaplastic potential of astrocytomas (Anticancer Res 1997, 17:2677-2682). Heat shock protein-coding genes have been found to have a putative AP-1 (activator protein-1)-binding site in their promoter region and the synthesis of these proteins is induced by the same extracellular stimuli that also activate AP-1 (a homo/heterodimer of members of the Jun and Fos families). In order to detect the putative relation of Hsp27 with AP-1 activation in human astrocytomas we examined eighty astrocytic tumors with different grades of malignancy for c-Jun, c-Fos, and Hsp27 expression. METHODS: Six pilocytic astrocytomas (WHO grade I), 15 diffuse fibrillary astrocytomas (WHO grade II), 19 anaplastic astrocytomas (WHO grade III), and 40 glioblastomas multiforme (WHO grade IV), were studied by immunohistochemistry using monoclonal and polyclonal antibodies directed against human Hsp27, c-Fos, and active (phosphorylated) forms of c-Jun (p-c-Jun). Monoclonal antibodies against the phosphorylated forms of the over-expressed MAP kinases JNK (c-Jun N-terminal kinase) (p-JNK) and p38 (p-p38) were also used. RESULTS: Overexpression of p-c-Jun, c-Fos and p-JNK was observed in the majority of glioblastomas (grade IV) whereas only minimal expression was noted in diffuse fibrillary astrocytomas (grade II). Hsp27 expression was observed only in the tumor specimens where c-Jun and c-Fos were co-expressed. AP-1/Hsp27 co-expression was associated with ascending grading of malignancy and it was independent of the proliferation index of the tumors. CONCLUSIONS: Our findings suggest that during malignant progression of astrocytomas there is activation of signal transduction cascade(s) culminating in AP-1 induction.
M Assimakopoulou; J Varakis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cancer research and clinical oncology     Volume:  127     ISSN:  0171-5216     ISO Abbreviation:  J. Cancer Res. Clin. Oncol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-21     Completed Date:  2002-01-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7902060     Medline TA:  J Cancer Res Clin Oncol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  727-32     Citation Subset:  IM    
Department of Anatomy, School of Medicine, University of Patras, Greece.
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MeSH Terms
Astrocytoma / chemistry*,  pathology
Child, Preschool
Heat-Shock Proteins / analysis*
JNK Mitogen-Activated Protein Kinases
Ki-67 Antigen / analysis
Middle Aged
Mitogen-Activated Protein Kinases / metabolism
Proto-Oncogene Proteins c-jun / analysis
Transcription Factor AP-1 / analysis*
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Ki-67 Antigen; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; EC Mitogen-Activated Protein Kinases; EC Protein Kinases

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