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ANP versus NT-proANP for the detection of left atrial dilatation in horses.
MedLine Citation:
PMID:  25257480     Owner:  NLM     Status:  Publisher    
REASONS FOR PERFORMING STUDY: Studies on the use of atrial natriuretic peptide (ANP) as a biomarker for left atrial dilatation in horses have produced variable results. Few have been performed and the results may have been influenced by ANP instability, differences in sampling protocol and changes in the assay over time. N-terminal proANP (NT-proANP), a more stable molecule, and might be a good alternative for clinical use.
OBJECTIVES: To compare ANP and NT-proANP in terms of the detection of left atrial dilatation and to determine the influence of sample storage at temperatures of -80°C and -20°C.
STUDY DESIGN: Prospective clinical study.
METHODS: ANP and NT-proANP concentrations in healthy horses (group 1, n = 20), horses with mitral valve regurgitation and a normal atrial size (group 2, n = 11) and horses with mitral valve regurgitation associated with left atrial dilatation (group 3, n = 16) were compared. ANP concentration was measured with an equine enzyme-linked immunosorbent assay (ELISA), and NT-proANP concentration with an ELISA developed for use in human patients. Samples were stored at -20°C and -80°C and analysed within 7 months.
RESULTS: The NT-proANP concentrations were not significantly different between the groups. Horses in group 3 had a significantly higher ANP concentration (median 366, interquartile range (IQR): 74-2000 pg/mL) compared to horses in groups 1 (median 31, IQR: 31-333 pg/mL) or 2 (median 31, IQR: 31-1152 pg/mL, P = 0.02). The ANP cut-off value for detection of left atrial dilatation was 52 pg/mL (sensitivity: 81%, specificity: 84%) for sample storage at -80°C, and 44 pg/mL (sensitivity: 69%, specificity: 84%) for storage at -20°C. A larger decrease in ANP (45 ± 126 pg/mL) than in NT-proANP (10 ± 31 pg/mL) was found associated with sample storage at -20°C instead of -80°C.
CONCLUSIONS: ANP, but not NT-proANP, can detect left atrial dilatation in horses. ANP is less stable than NT-proANP when samples are stored at -20°C. ANP is a more suitable biomarker of left atrial dilatation in horses than NT-proANP.
N Van Der Vekens; A Decloedt; D De Clercq; S Ven; S Sys; G van Loon
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-25
Journal Detail:
Title:  Equine veterinary journal     Volume:  -     ISSN:  2042-3306     ISO Abbreviation:  Equine Vet. J.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-26     Completed Date:  -     Revised Date:  2014-9-27    
Medline Journal Info:
Nlm Unique ID:  0173320     Medline TA:  Equine Vet J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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This article is protected by copyright. All rights reserved.
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