Document Detail

AMY-1 is a trigger for the erythrocyte differentiation of K562 cells.
MedLine Citation:
PMID:  10639579     Owner:  NLM     Status:  MEDLINE    
We have reported that a novel c-Myc binding protein, AMY-1, stimulated the transcription activity of c-Myc and was translocated from cytoplasm to nuclei in a c-Myc-dependent manner. Here, the role of AMY-1 in cell differentiation was examined. AMY-1 expression was up-regulated after differentiation induction of human K562 cells to erythrocyte cells by AraC, while c-Myc expression was rapidly down-regulated. K562 cell lines expressing exogenous AMY-1 were established, and these cells expressed a high level of epsilon-globin mRNA, a marker gene necessary for erythrocyte cell differentiation, without differentiation induction. The addition of AraC rapidly initiated differentiation in these cell lines, which continued to differentiate to erythrocyte cells possessing a high level of hemoglobin even after the decrease in AMY-1 expression. These results suggest that AMY-1 is a trigger for K562 cells to differentiate to erythrocyte cells and that AMY-1 may have a function independent of or different from c-Myc.
M Furusawa; T Onishi; T Taira; S M Iguchi-Ariga; H Ariga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  16     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-07     Completed Date:  2000-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  339-45     Citation Subset:  IM    
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
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MeSH Terms
Antimetabolites, Antineoplastic / pharmacology
Cell Differentiation / physiology*
Cytarabine / pharmacology
DNA-Binding Proteins / physiology*
Globins / metabolism
K562 Cells / drug effects,  metabolism*
RNA, Messenger / metabolism*
Transcription Factors / physiology*
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/DNA-Binding Proteins; 0/MYCBP protein, human; 0/RNA, Messenger; 0/Transcription Factors; 147-94-4/Cytarabine; 9004-22-2/Globins

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