Document Detail


AMPK supports growth in Drosophila by regulating muscle activity and nutrient uptake in the gut.
MedLine Citation:
PMID:  20478298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The larval phase of the Drosophila life cycle is characterized by constant food intake, resulting in a two hundred-fold increase in mass over four days. Here we show that the conserved energy sensor AMPK is essential for nutrient intake in Drosophila. Mutants lacking dAMPKalpha are small, with low triglyceride levels, small fat body cells and early pupal lethality. Using mosaic analysis, we find that dAMPKalpha functions as a nonautonomous regulator of cell growth. Nutrient absorption is impaired in dAMPKalpha mutants, and this defect stems not from altered gut epithelial cell polarity but from impaired peristaltic activity. Expression of a wild-type dAMPKalpha transgene or an activated version of the AMPK target myosin regulatory light chain (MRLC) in the dAMPKalpha mutant visceral musculature restores gut function and growth. These data suggest strongly that AMPK regulates visceral smooth muscle function through phosphorylation of MRLC. Furthermore, our data show that in Drosophila, AMPK performs an essential cell-nonautonomous function, serving the needs of the organism by promoting activity of the visceral musculature and, consequently, nutrient intake.
Authors:
Michelle L Bland; Robert J Lee; Julie M Magallanes; J Kevin Foskett; Morris J Birnbaum
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-05-15
Journal Detail:
Title:  Developmental biology     Volume:  344     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-23     Completed Date:  2010-08-10     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  293-303     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
AMP-Activated Protein Kinases / metabolism*
Animals
Crosses, Genetic
Drosophila melanogaster / metabolism*
Gene Expression Regulation, Developmental*
Glucose / metabolism
Intestines / embryology,  metabolism*
Models, Biological
Muscle, Smooth / metabolism
Muscles / embryology,  metabolism*
Peristalsis
Phosphorylation
Reverse Transcriptase Polymerase Chain Reaction
Transgenes
Triglycerides / metabolism
Grant Support
ID/Acronym/Agency:
DK068981/DK/NIDDK NIH HHS; P01 CA093615/CA/NCI NIH HHS; P01 CA093615-089002/CA/NCI NIH HHS; R01 DK056886/DK/NIDDK NIH HHS; R01 DK056886-12/DK/NIDDK NIH HHS; R01-DK56886/DK/NIDDK NIH HHS; R21 DK089391/DK/NIDDK NIH HHS; T32 DK007314/DK/NIDDK NIH HHS; T32 DK007314-27/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Triglycerides; EC 2.7.11.1/AMP-Activated Protein Kinases; IY9XDZ35W2/Glucose
Comments/Corrections

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