Document Detail

The AMP-activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-b-D-ribonucleoside, regulates lactate production in rat Sertoli cells.
MedLine Citation:
PMID:  17909267     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to investigate whether the AMP-activated protein kinase (AMPK), a key regulator of cellular energy homeostasis, is present in Sertoli cells and whether its activation by 5-aminoimidazole-4-carboxamide-1-b-d-ribonucleoside (AICAR) results in the regulation of cell metabolism to ensure lactate supply for germ cell development. Sertoli cell cultures from 20-day-old rats were used. Western blot analysis for the alpha-subunit of AMPK showed that high levels of AMPK are present in Sertoli cells. Treatment of the cultures with AICAR resulted in a dose- and time-dependent increase of P-AMPK levels indicating activation of the enzyme. A possible effect of AICAR on Sertoli cell lactate production was then analyzed. A dose- and time-dependent increment in lactate secretion was observed. The participation of AMPK activation in different biochemical processes that may be implicated in the regulation of lactate production was also analyzed. AICAR stimulated glucose uptake in a dose- and time-dependent manner. Additionally, AICAR increased the glucose transporter 1 (GLUT1) and decreased the glucose transporter 3 (GLUT3) mRNA levels. As for the role of AMPK in the regulation of the monocarboxylate transporters 1 and 4 (MCT1 and MCT4), it has been observed that AICAR treatment decreased MCT1 and increased MCT4 mRNA levels. In summary, the results presented herein show that AMPK is present in Sertoli cells and that its activation by AICAR increases lactate production as a result, at least in part, of a) an increase in glucose uptake, b) an increase in GLUT1 expression, and c) a decrease in MCT1 and an increase in MCT4 levels. Altogether, these results suggest an important role of AMPK in modulating the nutritional function of Sertoli cells.
María Noel Galardo; María Fernanda Riera; Eliana Herminia Pellizzari; Selva Beatriz Cigorraga; Silvina Beatriz Meroni
Related Documents :
8452927 - Cycle of the seminiferous epithelium in the djungarian hamster (phodopus sungorus sungo...
12628687 - Mouse leydig tumor cells produce c-19 steroids, including testosterone.
2256117 - The in vitro effects of four isomers of dinitrotoluene on rat sertoli and sertoli-germ ...
11352557 - Regulation of lactate production by fsh, il1beta, and tnfalpha in rat sertoli cells.
17516137 - Cartilage polysaccharide induces apoptosis in human leukemia k562 cells.
1312107 - Epidermal cell-polymorphonuclear leukocyte cooperation in the formation of leukotriene ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular endocrinology     Volume:  39     ISSN:  1479-6813     ISO Abbreviation:  J. Mol. Endocrinol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-02     Completed Date:  2007-11-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8902617     Medline TA:  J Mol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  279-88     Citation Subset:  IM    
Centro de Investigaciones Endocrinológicas (CEDIE), Hospital de Niños Ricardo Gutiérrez, Gallo 1330, 1425 Buenos Aires, Argentina.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
AMP-Activated Protein Kinases
ATP-Binding Cassette Transporters / genetics,  metabolism
Aminoimidazole Carboxamide / analogs & derivatives*,  pharmacology
Cells, Cultured
Enzyme Activators / pharmacology
Glucose / metabolism
L-Lactate Dehydrogenase / genetics,  metabolism
Lactic Acid / biosynthesis*
Monocarboxylic Acid Transporters / genetics,  metabolism
Multienzyme Complexes / metabolism*
Protein-Serine-Threonine Kinases / metabolism*
Rats, Sprague-Dawley
Ribonucleosides / pharmacology*
Sertoli Cells / drug effects*,  metabolism*
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Enzyme Activators; 0/Monocarboxylic Acid Transporters; 0/Multienzyme Complexes; 0/Ribonucleosides; 2627-69-2/acadesine; 360-97-4/Aminoimidazole Carboxamide; 50-21-5/Lactic Acid; 50-99-7/Glucose; EC Dehydrogenase; EC Protein Kinases; EC Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Crosstalk of CREB and Fos/Jun on a single cis-element: transcriptional repression of the steroidogen...
Next Document:  Role of specificity protein transcription factors in estrogen-induced gene expression in MCF-7 breas...