Document Detail

ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death.
MedLine Citation:
PMID:  19013425     Owner:  NLM     Status:  MEDLINE    
ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.
Berit R Høj; Jonas M la Cour; Jens Mollerup; Martin W Berchtold
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-14
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  378     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-08     Completed Date:  2008-12-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  145-8     Citation Subset:  IM    
Copenhagen Biocenter, Department of Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark.
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MeSH Terms
Apoptosis* / genetics
Apoptosis Regulatory Proteins / genetics,  physiology*
Calcium-Binding Proteins / genetics,  physiology*
Caspases / metabolism
Cell Division / genetics
G2 Phase / genetics
Gene Knockdown Techniques
Hela Cells
Neoplasms / enzymology*,  pathology
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Calcium-Binding Proteins; 0/PDCD6 protein, human; EC 3.4.22.-/Caspases

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