Document Detail


ALA-PDT results in phenotypic changes and decreased cellular invasion in surviving cancer cells.
MedLine Citation:
PMID:  19347942     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: The mechanisms of photodynamic therapy (PDT) have been studied on the cellular and tissue levels. However, the cellular behaviors of cancer cells survived from PDT are still not clear. This study attempted to investigate the influence of 5-aminolevulinic acid (ALA)-based PDT on the invasion ability as well as molecular changes in surviving cancer cells and their progeny. MATERIALS AND METHODS: The systematic effects of ALA-PDT were evaluated using human invasive carcinoma cells (lung adenocarcinoma CL1-5 cells, melanoma A375 cells and breast carcinoma MDA-MB-231 cells). To study the cellular behaviors of surviving cancer cells, PDT-derived variants were established as stable cell lines after consecutive treatment with ALA-PDT. Scratch wound assay and invasion assay were performed to evaluate the migration and invasion ability in the surviving cancer cells and the established PDT-derived variants. RT-PCR and immunoblot analysis were performed to examine the expression levels of epidermal growth factor receptor (EGFR). RESULTS: Though ALA-PDT caused differential phototoxicity among these invasive carcinoma cells, reduced migration was found in all the surviving cancer cells Compared to parental cancer cells, the established PDT-derived variants exerted significant phenotypic changes of cellular morphology, reduced mitochondrial function and a suppressed cellular invasiveness. Furthermore, correlated with the reduced invasion ability, expression of EGFR was downregulated in these established PDT-derived variants. CONCLUSIONS: Except for direct cell killing, ALA-PDT could reduce EGFR expression and invasion ability of the surviving cancer cells and these effects could further pass to the progeny. The results from this study provide insights into a new mechanism by which PDT might affect cellular behaviors and tumor metastasis.
Authors:
Tsuimin Tsai; Hong Tai Ji; Pei-Chi Chiang; Ruey-Hwang Chou; Wun-Shaing Wayne Chang; Chin-Tin Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  41     ISSN:  1096-9101     ISO Abbreviation:  Lasers Surg Med     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-09     Completed Date:  2009-07-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-15     Citation Subset:  IM    
Affiliation:
Graduate Institute of Biomedical Materials and Engineering, Graduate School of Dentistry, Taipei Medical University, Taipei 110, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / pathology,  radiotherapy
Aminolevulinic Acid / pharmacology*
Breast Neoplasms / pathology,  radiotherapy
Cell Line, Tumor
Cell Movement / radiation effects
Down-Regulation
Humans
Lung Neoplasms / pathology,  radiotherapy
Melanoma / pathology,  radiotherapy
Mitochondria / radiation effects
Neoplasm Invasiveness / genetics*
Phenotype
Photochemotherapy*
Receptor, Epidermal Growth Factor / metabolism,  radiation effects
Skin Neoplasms / pathology,  radiotherapy
Tumor Cells, Cultured / pathology*,  radiation effects*
Chemical
Reg. No./Substance:
106-60-5/Aminolevulinic Acid; EC 2.7.10.1/Receptor, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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