| AGS-3 alters Caenorhabditis elegans behavior after food deprivation via RIC-8 activation of the neural G protein G αo. | |
| | |
MedLine Citation:
|
PMID: 21832186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Proteins containing the G protein regulator (GPR) domain bind the major neural G protein Gα(o) in vitro. However, the biological functions of GPR proteins in neurons remain undefined, and based on the in vitro activities of GPR proteins it is unclear whether these proteins activate or inhibit G protein signaling in vivo. We found that the conserved GPR domain protein AGS-3 activates Gα(o) signaling in vivo to allow Caenorhabditis elegans to alter several behaviors after food deprivation, apparently so that the animals can more effectively seek food. AGS-3 undergoes a progressive change in its biochemical fractionation upon food deprivation, suggesting that effects of food deprivation are mediated by modifying this protein. We analyzed one C. elegans food-regulated behavior in depth; AGS-3 activates Gα(o) in the ASH chemosensory neurons to allow food-deprived animals to delay response to the aversive stimulus octanol. Genetic epistasis experiments show the following: (1) AGS-3 and the guanine nucleotide exchange factor RIC-8 act in ASH in a mutually dependent fashion to activate Gα(o); (2) this activation requires interaction of the GPR domains of AGS-3 with Gα(o); and (3) Gα(o)-GTP is ultimately the signaling molecule that acts in ASH to delay octanol response. These results identify a biological role for AGS-3 in response to food deprivation and indicate the mechanism for its activation of Gα(o) signaling in vivo. |
| | |
Authors:
|
Catherine Hofler; Michael R Koelle |
Related Documents
:
|
22361246 - Foodborne outbreaks of shigellosis in the usa, 1998-2008. 20958676 - Thought for food: suggestions for a systematised approach to emergency food distributio... 23276276 - Turning a blind eye to temptation: how cognitive load can facilitate self-regulation. 23144936 - Different levels of food restriction reveal genotype-specific differences in learning a... 23220356 - The marketing of foods and non-alcoholic beverages to children: setting the research ag... 8971536 - Treatment of men with flat (fc) or acuminata (ca) condylomata with interferon alpha-2a. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 31 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-08-11 Completed Date: 2011-10-04 Revised Date: 2012-11-12 |
Medline Journal Info:
|
Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
|
Languages: eng Pagination: 11553-62 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8024, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism, physiology* Cells, Cultured Drosophila Food Deprivation / physiology* GTP-Binding Protein alpha Subunits, Gi-Go / metabolism*, physiology Guanine Nucleotide Dissociation Inhibitors / metabolism, physiology* Humans Neurons / metabolism* Nuclear Proteins / metabolism*, physiology Protein Binding / genetics Signal Transduction / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
NS36918/NS/NINDS NIH HHS; R01 NS036918/NS/NINDS NIH HHS; R01 NS036918-14/NS/NINDS NIH HHS; R01 NS036918-15/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Caenorhabditis elegans Proteins; 0/GPSM1 protein, human; 0/Guanine Nucleotide Dissociation Inhibitors; 0/Nuclear Proteins; 0/RIC-8 protein, C elegans; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gi-Go |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nestin is not essential for development of the CNS but required for dispersion of acetylcholine rece...
Next Document: A new kinetic framework for synaptic vesicle trafficking tested in synapsin knock-outs.