Document Detail

AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans.
MedLine Citation:
PMID:  17488621     Owner:  NLM     Status:  MEDLINE    
Cell fusion is fundamental for reproduction and organ formation. Fusion between most C. elegans epithelial cells is mediated by the EFF-1 fusogen. However, fusion between the anchor cell and the utse syncytium that establishes a continuous uterine-vulval tube proceeds normally in eff-1 mutants. By isolating mutants where the anchor-cell fails to fuse, we identified aff-1. AFF-1 ectopic expression results in fusion of cells that normally do not fuse in C. elegans. The fusogen activity of AFF-1 was further confirmed by its ability to fuse heterologous cells. AFF-1 and EFF-1 differ in their fusogenic activity and expression patterns but share eight conserved predicted disulfide bonds in their ectodomains, including a putative TGF-beta-type-I-Receptor domain. We found that FOS-1, the Fos transcription factor ortholog that controls anchor-cell invasion during nematode development, is a specific activator of aff-1-mediated anchor-cell fusion. Thus, FOS-1 links cell invasion and fusion in a developmental cascade.
Amir Sapir; Jaebok Choi; Evgenia Leikina; Ori Avinoam; Clari Valansi; Leonid V Chernomordik; Anna P Newman; Benjamin Podbilewicz
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental cell     Volume:  12     ISSN:  1534-5807     ISO Abbreviation:  Dev. Cell     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-09     Completed Date:  2007-06-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  683-98     Citation Subset:  IM    
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
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MeSH Terms
Amino Acid Sequence
Caenorhabditis elegans / cytology*,  embryology,  metabolism*
Caenorhabditis elegans Proteins / chemistry,  metabolism*
Cell Fusion
Cytoplasm / metabolism
Embryo, Nonmammalian / cytology
Epithelial Cells / cytology
Insects / cytology
Models, Biological
Molecular Sequence Data
Mutation / genetics
Proto-Oncogene Proteins c-fos / chemistry,  metabolism*
Transcription Factors / chemistry,  metabolism*
Vulva / cytology,  growth & development
Grant Support
Z01 HD001501-14/HD/NICHD NIH HHS; Z01 HD001501-16/HD/NICHD NIH HHS
Reg. No./Substance:
0/AFF-1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/FOS-1 protein, C elegans; 0/Proto-Oncogene Proteins c-fos; 0/Transcription Factors
Comment In:
Dev Cell. 2007 May;12(5):667-8   [PMID:  17488618 ]

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